Key features and details
- Rabbit polyclonal to DHTKD1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DHTKD1 antibody
See all DHTKD1 primary antibodies
DescriptionRabbit polyclonal to DHTKD1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Orangutan
Recombinant fragment corresponding to Human DHTKD1 aa 1-280.
MASATAAAARRGLGRALPLFWRGYQTERGVYGYRPRKPESREPQGALERP PVDHGLARLVTVYCEHGHKAAKINPLFTGQALLENVPEIQALVQTLQGPF HTAGLLNMGKEEASLEEVLVYLNQIYCGQISIETSQLQSQDEKDWFAKRF EELQKETFTTEERKHLSKLMLESQEFDHFLATKFSTVKRYGGEGAESMMG FFHELLKMSAYSGITDVIIGMPHRGRLNLLTGLLQFPPELMFRKMRGLSE FPENFSATGDVLSHLTSSVDLYFGAHHPLH
Database link: Q96HY7
- WB: Mouse heart, liver and kidney tissue lysate; A431, MCF7 and HepG2 whole cell lysates. IHC-P: Human gastric cancer and brain tissue lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- HepG2 whole cell lysate (ab166833)
- Mouse liver tissue lysate - total protein (ab29301)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- MCF7 whole cell lysate (ab29537)
- A431 whole cell lysate (ab30132)
- Mouse heart normal tissue lysate - total protein (ab30291)
- A431 whole cell lysate (ab7909)
Our Abpromise guarantee covers the use of ab230392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionThe 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
Sequence similaritiesBelongs to the alpha-ketoglutarate dehydrogenase family.
- Information by UniProt
- AMOXAD antibody
- CMT2Q antibody
- Dehydrogenase E1 and transketolase domain containing 1 antibody
All lanes : Anti-DHTKD1 antibody (ab230392) at 1.82 µg/ml
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
All lanes : Goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDa
Paraffin-embedded human gastric cancer tissue stained for DHTKD1 using ab230392 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human brain tissue stained for DHTKD1 using ab230392 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230392 has not yet been referenced specifically in any publications.