Anti-DHX16 antibody (ab176819)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-DHX16 antibody -
Description
Rabbit polyclonal to DHX16 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human DHX16 aa 991-1041 (C terminal). The exact sequence is proprietary. NP_003578.1
Sequence:LTTKEFMRQV LEIESSWLLE VAPHYYKAKE LEDPHAKKMP KKIGKTREEL G
Database link: O60231 -
Positive control
- HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176819 was affinity purified using an epitope specific to DHX16 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab176819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-5 µg/mg of lysate. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 119 kDa. |
Target
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Function
Probable ATP-binding RNA helicase involved in pre-mRNA splicing. -
Sequence similarities
Belongs to the DEAD box helicase family. DEAH subfamily. DDX16/PRP8 sub-subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 100719266 Human
- Entrez Gene: 713836 Human
- Entrez Gene: 8449 Human
- Omim: 603405 Human
- SwissProt: O60231 Human
- Unigene: 485060 Human
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Alternative names
- 2410006N22Rik antibody
- ATP-dependent RNA helicase #3 antibody
- ATP-dependent RNA helicase 3 antibody
see all
Images
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Immunoprecipitation - Anti-DHX16 antibody (ab176819)Detection of DHX16 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176819 at 3 µg/mg lysate for IP (Lane 1). For WB detection ab176819 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.
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Western blot - Anti-DHX16 antibody (ab176819)All lanes : Anti-DHX16 antibody (ab176819) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 119 kDa
Exposure time: 10 seconds
Datasheets and documents
References
ab176819 has not yet been referenced specifically in any publications.
