Key features and details
- Rabbit polyclonal to DHX16
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DHX16 antibody
DescriptionRabbit polyclonal to DHX16
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
- HeLa whole cell lysate.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176819 was affinity purified using an epitope specific to DHX16 immobilized on solid support.
Our Abpromise guarantee covers the use of ab176819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 119 kDa.|
FunctionProbable ATP-binding RNA helicase involved in pre-mRNA splicing.
Sequence similaritiesBelongs to the DEAD box helicase family. DEAH subfamily. DDX16/PRP8 sub-subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
- Information by UniProt
- 2410006N22Rik antibody
- ATP-dependent RNA helicase #3 antibody
- ATP-dependent RNA helicase 3 antibody
Detection of DHX16 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176819 at 3 µg/mg lysate for IP (Lane 1). For WB detection ab176819 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.
All lanes : Anti-DHX16 antibody (ab176819) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 119 kDa
Exposure time: 10 seconds
ab176819 has not yet been referenced specifically in any publications.