Anti-DHX16 antibody (ab176819)
Key features and details
- Rabbit polyclonal to DHX16
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DHX16 antibody -
Description
Rabbit polyclonal to DHX16 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human DHX16 aa 991-1041 (C terminal). The exact sequence is proprietary. NP_003578.1
Sequence:LTTKEFMRQV LEIESSWLLE VAPHYYKAKE LEDPHAKKMP KKIGKTREEL G
Database link: O60231 -
Positive control
- HeLa whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176819 was affinity purified using an epitope specific to DHX16 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab176819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-5 µg/mg of lysate. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 119 kDa. |
Target
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Function
Probable ATP-binding RNA helicase involved in pre-mRNA splicing. -
Sequence similarities
Belongs to the DEAD box helicase family. DEAH subfamily. DDX16/PRP8 sub-subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 100719266 Human
- Entrez Gene: 713836 Human
- Entrez Gene: 8449 Human
- Omim: 603405 Human
- SwissProt: O60231 Human
- Unigene: 485060 Human
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Alternative names
- 2410006N22Rik antibody
- ATP-dependent RNA helicase #3 antibody
- ATP-dependent RNA helicase 3 antibody
see all
Images
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Immunoprecipitation - Anti-DHX16 antibody (ab176819)
Detection of DHX16 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176819 at 3 µg/mg lysate for IP (Lane 1). For WB detection ab176819 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.
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Western blot - Anti-DHX16 antibody (ab176819)All lanes : Anti-DHX16 antibody (ab176819) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 119 kDa
Exposure time: 10 seconds
Protocols
Datasheets and documents
References (0)
ab176819 has not yet been referenced specifically in any publications.
