Key features and details
- Rabbit polyclonal to DHX29
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DHX29 antibody
See all DHX29 primary antibodies
DescriptionRabbit polyclonal to DHX29
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Dog, Chimpanzee, Gorilla, Chinese hamster
Synthetic peptide corresponding to a region between residue 150 and 200 of Human DHX29 (NP_061903.2).
- HeLa and 293T cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500. Detects a band of approximately 175 kDa (predicted molecular weight: 155 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceDHX29 belongs to the DEAD box helicase family in the DEAH subfamily. It contains 1 helicase ATP-binding domain and 1 helicase C-terminal domain. DHX29 may be involved in ATP-dependent DNA or RNA unwinding.
Cellular localizationCytoplasmic (Probable)
- DDX29 antibody
- DEAH (Asp Glu Ala Asp/His) box polypeptide 29 antibody
- DEAH (Asp Glu Ala His) box polypeptide 29 antibody
All lanes : Anti-DHX29 antibody (ab70745) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 155 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
1mg whole cell lysate fro HeLa cells was immunoprecipitated using ab70745 at 3µg/mg lysate. For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab70745 at 1ug/ml. Detection: chemiluminescence with exposure time of 3 minutes.
ab70745 has been referenced in 1 publication.
- Murat P et al. RNA G-quadruplexes at upstream open reading frames cause DHX36- and DHX9-dependent translation of human mRNAs. Genome Biol 19:229 (2018). PubMed: 30591072