Key features and details
- Rabbit polyclonal to DHX30
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DHX30 antibody
See all DHX30 primary antibodies
DescriptionRabbit polyclonal to DHX30
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide, corresponding to a region between residues 1144 and 1194 of human DHX30 (NP_619520.1)
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab85754 was affinity purified using an epitope specific to DHX30 immobilized on solid support.
Our Abpromise guarantee covers the use of ab85754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).|
|IP||Use at 5-10 µg/mg of lysate.|
Sequence similaritiesBelongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
modificationsIsoform 2 is phosphorylated on Ser-15.
Cellular localizationCytoplasm and Mitochondrion. Cytoplasm. Mitochondrion matrix > mitochondrion nucleoid.
- Information by UniProt
- DDX30 antibody
- DEAH box protein 30 antibody
- Dhx30 antibody
All lanes : Anti-DHX30 antibody (ab85754) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 134 kDa
Observed band size: 134 kDa
Additional bands at: 110 kDa, 180 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Detection of Human DHX3 by Western Blot of Immunprecipitate.
All lanes: ab85754 at 0.4µg/ml staining DHX3 in HeLa whole cell lysates immunoprecipitated at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using the following antibodies:
Lane 1: ab85754
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 3 minutes.
ab85754 has not yet been referenced specifically in any publications.