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Rabbit polyclonal to DHX30
Predicted to work with:
Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide, corresponding to a region between residues 1144 and 1194 of human DHX30 (NP_619520.1)
HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab85754 was affinity purified using an epitope specific to DHX30 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
Use at 5-10 µg/mg of lysate.
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Isoform 2 is phosphorylated on Ser-15.
Cytoplasm and Mitochondrion. Cytoplasm. Mitochondrion matrix > mitochondrion nucleoid.
Information by UniProt
DEAH box protein 30 antibody
Western blot - Anti-DHX30 antibody (ab85754)
All lanes : Anti-DHX30 antibody (ab85754) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Predicted band size: 134 kDa Observed band size: 134 kDa Additional bands at: 110 kDa, 180 kDa. We are unsure as to the identity of these extra bands. Exposure time: 3 minutes
Immunoprecipitation - Anti-DHX30 antibody (ab85754)
Detection of Human DHX3 by Western Blot of Immunprecipitate.
All lanes: ab85754 at 0.4µg/ml staining DHX3 in HeLa whole cell lysates immunoprecipitated at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using the following antibodies: Lane 1: ab85754 Lane 2: Control IgG Detection: Chemiluminescence with exposure time of 3 minutes.
has not yet been referenced specifically in any publications.
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