Anti-DHX33 antibody (ab72451)
Key features and details
- Rabbit polyclonal to DHX33
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DHX33 antibody
See all DHX33 primary antibodies -
Description
Rabbit polyclonal to DHX33 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Rhesus monkey, Orangutan, Elephant -
Immunogen
Synthetic peptide within Human DHX33 aa 650 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_064547.2
Database link: Q9H6R0 -
Positive control
- Hela whole cell lysate; 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity purified using an epitope specific to DHX33 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
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IP |
Use a concentration of 2 - 5 µg/ml.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa). |
IP
Use a concentration of 2 - 5 µg/ml. |
Target
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Relevance
DHX33 is a DEAD box protein whose function has not yet been determined. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. -
Cellular localization
Nucleus, nucleolus. -
Database links
- Entrez Gene: 56919 Human
- SwissProt: Q9H6R0 Human
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Alternative names
- DDX33 antibody
- DEAD box polypeptide 33 antibody
- DEAD/H (Asp Glu Ala Asp/His) box polypeptide 33 antibody
see all
Images
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All lanes : Anti-DHX33 antibody (ab72451) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 79 kDa
Observed band size: 79 kDa
Additional bands at: 200 kDa, 31 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunoprecipitation of DHX33 from 1mg whole HeLa cells lysate by 3 ug ab72451 followed by WB (20% of IP loaded):
Lane 1: ab72451 precipitate
Lane 2: Control IgG
For blotting immunoprecipitated DHX33, ab72451 was used at 1 ug/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab72451 has not yet been referenced specifically in any publications.