Key features and details
- Rabbit polyclonal to DHX33
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DHX33 antibody
See all DHX33 primary antibodies
DescriptionRabbit polyclonal to DHX33
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Rhesus monkey, Orangutan, Elephant
A synthetic peptide corresponding to a region between residues 650 and the C terminus (residue 707) of human DHX33.
- Hela whole cell lysate; 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified using an epitope specific to DHX33 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Our Abpromise guarantee covers the use of ab72451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).|
|IP||Use a concentration of 2 - 5 µg/ml.|
RelevanceDHX33 is a DEAD box protein whose function has not yet been determined. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
Cellular localizationNucleus, nucleolus.
- DDX33 antibody
- DEAD box polypeptide 33 antibody
- DEAD/H (Asp Glu Ala Asp/His) box polypeptide 33 antibody
All lanes : Anti-DHX33 antibody (ab72451) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 79 kDa
Observed band size: 79 kDa
Additional bands at: 200 kDa, 31 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Immunoprecipitation of DHX33 from 1mg whole HeLa cells lysate by 3 ug ab72451 followed by WB (20% of IP loaded):
Lane 1: ab72451 precipitate
Lane 2: Control IgG
For blotting immunoprecipitated DHX33, ab72451 was used at 1 ug/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.
ab72451 has not yet been referenced specifically in any publications.