Anti-DHX35 antibody (ab235366)
Key features and details
- Rabbit polyclonal to DHX35
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DHX35 antibody
See all DHX35 primary antibodies -
Description
Rabbit polyclonal to DHX35 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Orangutan -
Immunogen
Recombinant fragment corresponding to Human DHX35 aa 1-300.
Database link: Q9H5Z1 -
Positive control
- WB: Mouse kidney and liver lysate; K562 and HeLa cell lysates. IHC-P: Human brain and testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 79 kDa.
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 79 kDa. |
IHC-P
1/20 - 1/200. |
Target
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Relevance
DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The function of DHX35 which is a member of this family, has not been determined.DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The function of this gene product which is a member of this family, has not been determined. -
Cellular localization
Spliceosomal complex -
Database links
- Entrez Gene: 60625 Human
- Entrez Gene: 71715 Mouse
- SwissProt: Q9H5Z1 Human
- SwissProt: Q5RBD4 Orangutan
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Alternative names
- C20orf15 antibody
- DDX35 antibody
- DEAH (Asp-Glu-Ala-His) box polypeptide 35 antibody
see all
Images
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Paraffin-embedded human testis tissue stained for DHX35 using ab235366 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-DHX35 antibody (ab235366) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse liver lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 79 kDa -
Paraffin-embedded human brain tissue stained for DHX35 using ab235366 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235366 has not yet been referenced specifically in any publications.