Overview

  • Product name

  • Description

    Rabbit polyclonal to DHX35
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human DHX35 aa 1-300.
    Sequence:

    MAAPVGPVKFWRPGTEGPGVSISEERQSLAENSGTTVVYNPYAALSIEQQ RQKLPVFKLRNHILYLIENYQTVVIVGETGCGKSTQIPQYLAEAGWTAEG RVVGVTQPRRVAAVTVAGRVAEERGAVLGHEVGYCIRFDDCTDQLATRIK FLTDGMLVREMMVDPLLTKYSVIMLDEAHERTLYTDIAIGLLKKIQKKRG DLRLIVASATLDADKFRDFFNQNETSDPARDTCVILTVEGRTFPVDIFYL QSPVPDYIKSTVETVVKIHQTEGDGDVLAFLTGQEEVETVVSMLIEQARA


    Database link: Q9H5Z1

  • Positive control

    • WB: Mouse kidney and liver lysate; K562 and HeLa cell lysates. IHC-P: Human brain and testis tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab235366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 79 kDa.
IHC-P 1/20 - 1/200.

Target

  • Relevance

    DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The function of DHX35 which is a member of this family, has not been determined.DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The function of this gene product which is a member of this family, has not been determined.
  • Cellular localization

    Spliceosomal complex
  • Database links

  • Alternative names

    • C20orf15 antibody
    • DDX35 antibody
    • DEAH (Asp-Glu-Ala-His) box polypeptide 35 antibody
    • DEAH box protein 35 antibody
    • KAIA0875 antibody
    see all

Images

  • Paraffin-embedded human testis tissue stained for DHX35 using ab235366 at 1/100 dilution in immunohistochemical analysis.

  • All lanes : Anti-DHX35 antibody (ab235366) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Mouse liver lysate
    Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 79 kDa

  • Paraffin-embedded human brain tissue stained for DHX35 using ab235366 at 1/100 dilution in immunohistochemical analysis.

References

ab235366 has not yet been referenced specifically in any publications.

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