Product nameAnti-DHX36 antibody
See all DHX36 primary antibodies
DescriptionRabbit polyclonal to DHX36
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human DHX36 (N terminal). The exact sequence is proprietary.
Database link: Q9H2U1
- WB: HEK-293T, A431, HeLa and HepG2 cell lysate. IHC: Mouse testis and rat brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 115 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionPlays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. May function in sex development and spermatogenesis.
Tissue specificityHighly expressed in testis.
Sequence similaritiesBelongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localizationNucleus. Cytoplasm. Isoform 1 preferentially localized to the nucleus and isoform 2 localized to the cytoplasm. However, partitioning of cellular localization between the nucleus and cytoplasm is not exclusive, as isoform 1 was also detected in the cytoplasm. Both isoforms were excluded from nucleoli.
- Information by UniProt
- DDX36 antibody
- DEAH box protein 36 antibody
- Dhx36 antibody
All lanes : Anti-DHX36 antibody (ab226813) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 115 kDa
Paraffin-embedded mouse testis tissue stained for DHX36 using ab226813 at 1/400 dilution in immunohistochemical analysis.
Paraffin-embedded rat brain tissue stained for DHX36 using ab226813 at 1/400 dilution in immunohistochemical analysis.
ab226813 has not yet been referenced specifically in any publications.