Overview

  • Product name

  • Description

    Rabbit polyclonal to DHX36
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human DHX36 (N terminal). The exact sequence is proprietary.
    Database link: Q9H2U1

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 cell lysate. IHC: Mouse testis and rat brain tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 115 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. May function in sex development and spermatogenesis.
  • Tissue specificity

    Highly expressed in testis.
  • Sequence similarities

    Belongs to the DEAD box helicase family. DEAH subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization

    Nucleus. Cytoplasm. Isoform 1 preferentially localized to the nucleus and isoform 2 localized to the cytoplasm. However, partitioning of cellular localization between the nucleus and cytoplasm is not exclusive, as isoform 1 was also detected in the cytoplasm. Both isoforms were excluded from nucleoli.
  • Information by UniProt
  • Database links

  • Alternative names

    • DDX36 antibody
    • DEAH box protein 36 antibody
    • Dhx36 antibody
    • DHX36_HUMAN antibody
    • EC 3.6.1.- antibody
    • KIAA1488 antibody
    • MLE like protein 1 antibody
    • MLE-like protein 1 antibody
    • MLEL1 antibody
    • Probable ATP dependent RNA helicase DHX36 antibody
    • Probable ATP-dependent RNA helicase DHX36 antibody
    • RHAU antibody
    • RNA helicase associated with AU rich element ARE antibody
    • RNA helicase associated with AU-rich element ARE antibody
    see all

Images

  • All lanes : Anti-DHX36 antibody (ab226813) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 115 kDa

  • Paraffin-embedded mouse testis tissue stained for DHX36 using ab226813 at 1/400 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat brain tissue stained for DHX36 using ab226813 at 1/400 dilution in immunohistochemical analysis.

References

ab226813 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226813.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up