Anti-DHX36 antibody (ab70269)
Key features and details
- Rabbit polyclonal to DHX36
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DHX36 antibody
See all DHX36 primary antibodies -
Description
Rabbit polyclonal to DHX36 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rhesus monkey, Gorilla, Orangutan -
Immunogen
A region corresponding to C terminal amino acids 950-1008 of Human DHX36 (NP_065916.1)
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Positive control
- WB: HeLa, HEK293T, Jurkat, mouse NIH3T3, TCKM, Renca whole cell lysates; IHC: human testicular seminoma, human testis;
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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WB | (1) |
1/5000 - 1/15000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
1/5000 - 1/15000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. May function in sex development and spermatogenesis. -
Tissue specificity
Highly expressed in testis. -
Sequence similarities
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Nucleus. Cytoplasm. Isoform 1 preferentially localized to the nucleus and isoform 2 localized to the cytoplasm. However, partitioning of cellular localization between the nucleus and cytoplasm is not exclusive, as isoform 1 was also detected in the cytoplasm. Both isoforms were excluded from nucleoli. - Information by UniProt
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Database links
- Entrez Gene: 170506 Human
- Entrez Gene: 72162 Mouse
- Omim: 612767 Human
- SwissProt: Q9H2U1 Human
- SwissProt: Q8VHK9 Mouse
- Unigene: 446270 Human
- Unigene: 224233 Mouse
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Alternative names
- DDX36 antibody
- DEAH box protein 36 antibody
- Dhx36 antibody
see all
Images
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All lanes : Anti-DHX36 antibody (ab70269) at 1 µg/ml
Lane 1 : NIH/3T3 whole cell lysate
Lane 2 : TCKM whole cell lysate
Lane 3 : Renca whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 115 kDa -
All lanes : Anti-DHX36 antibody (ab70269) at 0.4 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HEK293T cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Predicted band size: 115 kDa -
Immunoprecipitation of HeLa whole cell lysate using ab70269
Lane 1: current lot ab70269
Lane 2: previous lot ab70269
Lane 3: Control IgG
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling DHX36 with ab70269 at 1/1000 (1µg/ml). Detection: DAB.
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IHC image of ab70269 staining in Human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70269, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab70269 has been referenced in 12 publications.
- Liu H et al. A Helicase Unwinds Hexanucleotide Repeat RNA G-Quadruplexes and Facilitates Repeat-Associated Non-AUG Translation. J Am Chem Soc 143:7368-7379 (2021). PubMed: 33855846
- He L et al. DNA sensors, crucial receptors to resist pathogens, are deregulated in colorectal cancer and associated with initiation and progression of the disease. J Cancer 11:893-905 (2020). PubMed: 31949493
- Herviou P et al. hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma. Nat Commun 11:2661 (2020). PubMed: 32461552
- Murat P et al. RNA G-quadruplexes at upstream open reading frames cause DHX36- and DHX9-dependent translation of human mRNAs. Genome Biol 19:229 (2018). PubMed: 30591072
- Remenyi J et al. The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132. Sci Rep 6:22848 (2016). PubMed: 26947125