Product nameAnti-DHX37 antibody
DescriptionRabbit polyclonal to DHX37
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Guinea pig, Cow, Pig, Rhesus monkey, Gorilla, Elephant
Synthetic peptide corresponding to a region between residue 1 and 50 of human DHX37 (NP_116045.2).
- HeLa, 293T and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70778 was affinity purified using an epitope specific to DHX37 immobilized on solid support.
Our Abpromise guarantee covers the use of ab70778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceDHX37 is a member of the family of the DEAD box proteins, a group of proteins that present the conserved motif Asp-Glu-Ala-Asp (DEAD). They have a role in RNA translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.
- DDX37 antibody
- DEAH (Asp-Glu-Ala-His) box polypeptide 37 antibody
- DEAH box protein 37 antibody
All lanes : Anti-DHX37 antibody (ab70778) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 130 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Detection of DHX37 by Western Blot of Immunprecipitate. ab70778 at 1µg/ml staining DHX37 in HeLa whole cell lysate immunoprecipitated using ab70778 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.