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Rabbit polyclonal to DHX38
Predicted to work with:
Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to a region between residue 1200 and the C-terminus (residue 1227) of human DHX38 (NP_054722.2).
HeLa, 293T and NIH3T3 whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab70781 was affinity purified using an epitope specific to DHX38 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 140 kDa).
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Probable ATP-binding RNA helicase involved in pre-mRNA splicing.
Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.
Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Information by UniProt
ATP dependent RNA helicase DHX38 antibody
ATP-dependent RNA helicase DHX38 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX38 antibody (ab70781)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DHX38 with ab70781 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Anti-DHX38 antibody (ab70781)
All lanes : Anti-DHX38 antibody (ab70781) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg Predicted band size: 140 kDa Observed band size: 140 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-DHX38 antibody (ab70781)
Detection of DHX38 by Western Blot of Immunprecipitate.
ab70781 at 1µg/ml staining DHX38 in HeLa whole cell lysate immunoprecipitated using ab70781 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
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