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Rabbit polyclonal to DHX57
Predicted to work with:
Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 150 and 200 of human DHX57 (NP_945314.1).
HeLa and 293T whole cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab86784 was affinity purified using an epitope specific to DHX57 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 10 µg/mg of lysate.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 156 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Probable ATP-binding RNA helicase.
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 1 C3H1-type zinc finger. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 UBA domain.
Information by UniProt
DEAH (Asp Glu Ala Asp/His) box polypeptide 57 antibody
DEAH box protein 57 antibody
Western blot - Anti-DHX57 antibody (ab86784)
All lanes :
Anti-DHX57 antibody (ab86784) at 0.04 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Lane 4 :
293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size:
Observed band size:
171 kDa (
why is the actual band size different from the predicted?
Immunoprecipitation - Anti-DHX57 antibody (ab86784)
Detection of DHX57 by Western Blot of Immunprecipitate.
ab86784, at 0.4 µg/ml, staining DHX57 in HeLa whole cell lysate immunoprecipitated using ab86784 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"