Product nameAnti-DHX58/RLR antibody
See all DHX58/RLR primary antibodies
DescriptionRabbit polyclonal to DHX58/RLR
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human DHX58/RLR (C terminal).
- Rat kidney tissue lysate. IF/ICC: HepG2 cell line.
Previously labelled as DHX58.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab67270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionParticipates in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade which results in the induction and expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). The RNA helicase domain may recognize and structurally modify viral RNA to facilitate detection by DDX58/RIG-I or by IFIH1/MDA5 whose affinity for dsRNA is lower.
Sequence similaritiesBelongs to the helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
- Information by UniProt
- D11LGP2 antibody
- D11lgp2e antibody
- DEXH (Asp Glu X His) box polypeptide 58 antibody
ICC/IF image of ab67270 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67270, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Lane 1 : Anti-DHX58/RLR antibody (ab67270) at 1 µg/ml
Lane 2 : Anti-DHX58/RLR antibody (ab67270) at 2 µg/ml
All lanes : Rat
kidney tissue lysate
Lysates/proteins at 15 µg per lane.
All lanes : Anti rabbit secondary antibody
Predicted band size: 77 kDa
Observed band size: 77 kDa
Additional bands at: 80 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Childs KS et al. LGP2 plays a critical role in sensitizing mda-5 to activation by double-stranded RNA. PLoS One 8:e64202 (2013). WB ; Human . Read more (PubMed: 23671710) »
- Childs K et al. Paramyxovirus V proteins interact with the RNA Helicase LGP2 to inhibit RIG-I-dependent interferon induction. J Virol 86:3411-21 (2012). WB . Read more (PubMed: 22301134) »