Key features and details
- Rabbit polyclonal to DHX58/RLR
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-DHX58/RLR antibody
See all DHX58/RLR primary antibodies
DescriptionRabbit polyclonal to DHX58/RLR
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human DHX58/RLR (C terminal).
Previously labelled as DHX58.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab67270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).|
FunctionParticipates in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade which results in the induction and expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). The RNA helicase domain may recognize and structurally modify viral RNA to facilitate detection by DDX58/RIG-I or by IFIH1/MDA5 whose affinity for dsRNA is lower.
Sequence similaritiesBelongs to the helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
- Information by UniProt
- D11LGP2 antibody
- D11lgp2e antibody
- DEXH (Asp Glu X His) box polypeptide 58 antibody
Lane 1 : Anti-DHX58/RLR antibody (ab67270) at 1 µg/ml
Lane 2 : Anti-DHX58/RLR antibody (ab67270) at 2 µg/ml
All lanes : Rat
kidney tissue lysate
Lysates/proteins at 15 µg per lane.
All lanes : Anti rabbit secondary antibody
Predicted band size: 77 kDa
Observed band size: 77 kDa
Additional bands at: 80 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
ab67270 has been referenced in 4 publications.
- Yamane D et al. Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses. Nat Microbiol 4:1096-1104 (2019). PubMed: 30988429
- Parisien JP et al. RNA sensor LGP2 inhibits TRAF ubiquitin ligase to negatively regulate innate immune signaling. EMBO Rep 19:N/A (2018). PubMed: 29661858
- Childs KS et al. LGP2 plays a critical role in sensitizing mda-5 to activation by double-stranded RNA. PLoS One 8:e64202 (2013). WB ; Human . PubMed: 23671710
- Childs K et al. Paramyxovirus V proteins interact with the RNA Helicase LGP2 to inhibit RIG-I-dependent interferon induction. J Virol 86:3411-21 (2012). WB . PubMed: 22301134