Anti-DHX58/RLR antibody (ab67270)
Key features and details
- Rabbit polyclonal to DHX58/RLR
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DHX58/RLR antibody
See all DHX58/RLR primary antibodies -
Description
Rabbit polyclonal to DHX58/RLR -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human DHX58/RLR (C terminal).
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General notes
Previously labelled as DHX58.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab67270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). |
Target
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Function
Participates in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade which results in the induction and expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). The RNA helicase domain may recognize and structurally modify viral RNA to facilitate detection by DDX58/RIG-I or by IFIH1/MDA5 whose affinity for dsRNA is lower. -
Sequence similarities
Belongs to the helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 79132 Human
- Entrez Gene: 80861 Mouse
- Entrez Gene: 303538 Rat
- Omim: 608588 Human
- SwissProt: Q96C10 Human
- SwissProt: Q99J87 Mouse
- Unigene: 55918 Human
- Unigene: 271830 Mouse
see all -
Alternative names
- D11LGP2 antibody
- D11lgp2e antibody
- DEXH (Asp Glu X His) box polypeptide 58 antibody
see all
Images
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Western blot - Anti-DHX58/RLR antibody (ab67270)Lane 1 : Anti-DHX58/RLR antibody (ab67270) at 1 µg/ml
Lane 2 : Anti-DHX58/RLR antibody (ab67270) at 2 µg/ml
All lanes : Rat
kidney tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti rabbit secondary antibody
Predicted band size: 77 kDa
Observed band size: 77 kDa
Additional bands at: 80 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
References (4)
ab67270 has been referenced in 4 publications.
- Yamane D et al. Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses. Nat Microbiol 4:1096-1104 (2019). PubMed: 30988429
- Parisien JP et al. RNA sensor LGP2 inhibits TRAF ubiquitin ligase to negatively regulate innate immune signaling. EMBO Rep 19:N/A (2018). PubMed: 29661858
- Childs KS et al. LGP2 plays a critical role in sensitizing mda-5 to activation by double-stranded RNA. PLoS One 8:e64202 (2013). WB ; Human . PubMed: 23671710
- Childs K et al. Paramyxovirus V proteins interact with the RNA Helicase LGP2 to inhibit RIG-I-dependent interferon induction. J Virol 86:3411-21 (2012). WB . PubMed: 22301134
