Recombinant Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9146(2)] to DHX8/prp22
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-DHX8/prp22 antibody [EPR9146(2)]
See all DHX8/prp22 primary antibodies -
Description
Rabbit monoclonal [EPR9146(2)] to DHX8/prp22 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Y79, heLa, RAW 264.7, and PC-12 cell lysates and Jurkat whole cell lysate (ab7899);
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9146(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/20000. Detects a band of approximately 139 kDa (predicted molecular weight: 139 kDa).
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Notes |
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WB
1/1000 - 1/20000. Detects a band of approximately 139 kDa (predicted molecular weight: 139 kDa). |
Target
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Function
Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. -
Sequence similarities
Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 S1 motif domain. -
Domain
The RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1659 Human
- Entrez Gene: 217207 Mouse
- Entrez Gene: 287727 Rat
- Omim: 600396 Human
- SwissProt: Q14562 Human
- SwissProt: A2A4P0 Mouse
- Unigene: 463105 Human
- Unigene: 28186 Mouse
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Alternative names
- ATP dependent RNA helicase DHX8 antibody
- ATP-dependent RNA helicase dhx8 antibody
- DDX 8 antibody
see all
Images
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All lanes : Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 139 kDa
Observed band size: 139 kDa -
Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074) at 1/5000 dilution + Y79 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 139 kDa
Exposure time: 30 seconds -
Anti-DHX8/prp22 antibody [EPR9146(2)] (ab181074) at 1/20000 dilution + Jurkat cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 139 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab181074 has been referenced in 1 publication.
- Chen X et al. High Levels of DEAH-Box Helicases Relate to Poor Prognosis and Reduction of DHX9 Improves Radiosensitivity of Hepatocellular Carcinoma. Front Oncol 12:900671 (2022). PubMed: 35814441