Key features and details
- Rabbit polyclonal to DHX8/prp22
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DHX8/prp22 antibody
See all DHX8/prp22 primary antibodies
DescriptionRabbit polyclonal to DHX8/prp22
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Tilapia, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to Human DHX8/prp22 aa 325-375.
Database link: NP_004932.1
- Whole cell lysate from HeLa and 293T cells.
This product was previously labelled as DHX8
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).|
|IP||Use at 1-4 µg/mg of lysate.|
FunctionFacilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.
Sequence similaritiesBelongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 S1 motif domain.
DomainThe RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome.
- Information by UniProt
- ATP dependent RNA helicase DHX8 antibody
- ATP-dependent RNA helicase dhx8 antibody
- DDX 8 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma (right) tissues labelling DHX8/prp22 with ab70588 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-DHX8/prp22 antibody (ab70588) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 139 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: <30 kDa, 117 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation of DHX8/prp22 from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab70588 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent WB detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab70588 has been referenced in 1 publication.
- Vogel Ciernia A et al. Mutation of neuron-specific chromatin remodeling subunit BAF53b: rescue of plasticity and memory by manipulating actin remodeling. Learn Mem 24:199-209 (2017). PubMed: 28416631