Key features and details
- Rabbit polyclonal to DIAPH1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DIAPH1 antibody
See all DIAPH1 primary antibodies
DescriptionRabbit polyclonal to DIAPH1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment, corresponding to a sequence within amino acids 1-207 of Human DIAPH1
- H1299 and Raji whole cell lysates. HeLa cells
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 141 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionActs in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
Tissue specificityExpressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea.
Involvement in diseaseDefects in DIAPH1 are the cause of deafness autosomal dominant type 1 (DFNA1) [MIM:124900]. DFNA1 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Sequence similaritiesBelongs to the formin homology family. Diaphanous subfamily.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
DomainDRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction (By similarity). DCAF7 binds to the FH2 (formin homology 2) domain.
Cellular localizationCell membrane. Cell projection > ruffle membrane. Cytoplasm > cytoskeleton. Membrane ruffles, especially at the tip of ruffles, of motile cells.
- Information by UniProt
- DIAPH1 antibody
- deafness, autosomal dominant 1 antibody
- DFNA1 antibody
All lanes : Anti-DIAPH1 antibody (ab96784) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 141 kDa
ab96784 at 1/200 dilution staining DIAPH1 in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is co-stained with Hoechst 33342.
ab96784 has been referenced in 4 publications.
- Ninoyu Y et al. The integrity of cochlear hair cells is established and maintained through the localization of Dia1 at apical junctional complexes and stereocilia. Cell Death Dis 11:536 (2020). PubMed: 32678080
- Latham SL et al. Variants in exons 5 and 6 of ACTB cause syndromic thrombocytopenia. Nat Commun 9:4250 (2018). PubMed: 30315159
- Wakayama S et al. Chemical labelling for visualizing native AMPA receptors in live neurons. Nat Commun 8:14850 (2017). PubMed: 28387242
- Chugh P et al. Actin cortex architecture regulates cell surface tension. Nat Cell Biol 19:689-697 (2017). WB ; Mouse . PubMed: 28530659