Overview

  • Product name

    Anti-DIAPH1 antibody [EPR7948]
    See all DIAPH1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7948] to DIAPH1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DIAPH1 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • 293T, HeLa, K562 and Human fetal brain lysates; Human stomach tissue; HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 141 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
    • Tissue specificity

      Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea.
    • Involvement in disease

      Defects in DIAPH1 are the cause of deafness autosomal dominant type 1 (DFNA1) [MIM:124900]. DFNA1 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
    • Sequence similarities

      Belongs to the formin homology family. Diaphanous subfamily.
      Contains 1 DAD (diaphanous autoregulatory) domain.
      Contains 1 FH1 (formin homology 1) domain.
      Contains 1 FH2 (formin homology 2) domain.
      Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
    • Domain

      DRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction (By similarity). DCAF7 binds to the FH2 (formin homology 2) domain.
    • Cellular localization

      Cell membrane. Cell projection > ruffle membrane. Cytoplasm > cytoskeleton. Membrane ruffles, especially at the tip of ruffles, of motile cells.
    • Information by UniProt
    • Database links

    • Alternative names

      • DIAPH1 antibody
      • deafness, autosomal dominant 1 antibody
      • DFNA1 antibody
      • DIA1 antibody
      • DIAP1 antibody
      • DIAP1_HUMAN antibody
      • DIAPH1 antibody
      • Diaphanous homolog 1 (Drosophila) antibody
      • diaphanous homolog 1 antibody
      • Diaphanous related formin 1 antibody
      • Diaphanous-related formin-1 antibody
      • DRF1 antibody
      • FLJ25265 antibody
      • hDIA1 antibody
      • LFHL1 antibody
      • low frequency hearing loss 1 antibody
      • p140DIA antibody
      • Protein diaphanous homolog 1 antibody
      see all

    Images

    • All lanes : Anti-DIAPH1 antibody [EPR7948] (ab129167) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Human fetal brain lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 141 kDa

    • ab129167, at 1/100 dilution, staining DIAPH1 in HeLa cells by Immunofluorescence.
    • ab129167, at 1/50 dilution staining DIAPH1 in paraffin-embedded human stomach tissue, by Immunohistochemistry.

    References

    This product has been referenced in:

    • Yang J  et al. DIAPH1 Is Upregulated and Inhibits Cell Apoptosis through ATR/p53/Caspase-3 Signaling Pathway in Laryngeal Squamous Cell Carcinoma. Dis Markers 2019:6716472 (2019). Read more (PubMed: 30733838) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (4-12% gradient Bis Tris gel ran 1X MOPS)
    Loading amount
    10 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Abcam user community

    Verified customer

    Submitted Nov 05 2018

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