Overview

  • Product name
  • Description
    Rabbit polyclonal to DIAPH2
  • Host species
    Rabbit
  • Specificity
    This antibody detects a band at 130kD in HEK293 cells representing DIA2.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    VPLERSRSRHNGAISSK

    conjugated to KLH, corresponding to amino acids 1085-1101 of Human DIA.

  • Positive control
    • HEK293 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab10562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/6000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).

Target

  • Function
    Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.
  • Tissue specificity
    Expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes.
  • Involvement in disease
    Defects in DIAPH2 are the cause of premature ovarian failure type 2A (POF2A) [MIM:300511]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similarities
    Belongs to the formin homology family. Diaphanous subfamily.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • Developmental stage
    Expressed from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles.
  • Domain
    DRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
  • Cellular localization
    Cytoplasm > cytosol. Early endosome. Isoform 3 is cytosolic but when coexpressed with RHOD, the 2 proteins colocalize to early endosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dia 2 antibody
    • DIA antibody
    • Dia drome antibody
    • Dia2 antibody
    • Diap 2 antibody
    • Diap2 antibody
    • DIAP2_HUMAN antibody
    • DIAPH 2 antibody
    • Diaph2 antibody
    • Diaphanous 2 antibody
    • Diaphanous homolog 2 (Drosophila) antibody
    • Diaphanous homolog 2 antibody
    • Diaphanous related formin 2 antibody
    • Diaphanous-related formin-2 antibody
    • Diaphanous2 antibody
    • Diaphorase 2 antibody
    • Diaphorase2 antibody
    • DRF 2 antibody
    • DRF2 antibody
    • FLJ11167 antibody
    • OTTHUMP00000024270 antibody
    • OTTHUMP00000024271 antibody
    • OTTHUMP00000062171 antibody
    • POF 2 antibody
    • POF antibody
    • POF2 antibody
    • Protein diaphanous homolog 2 antibody
    see all

Images

  • Western blot using ab10562 on HEK293 whole cell lysate at 1/500.

    Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
    Exposure time: 1 minute
    Expected molecular weight: 132 kDa
    20µg of cell lysate per lane

    Western blot using ab10562 on HEK293 whole cell lysate at 1/500. Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000) Exposure time: 1 minute Expected molecular weight: 132 kDa 20µg of cell lysate per lane

References

ab10562 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your recent e-mail. I have looked into your concerns very carefully and agree with you that the terminology can be very confusing. Following a BLAST search with the immunogen I can confirm the following: The immunogen sequence used to generate the antibody has 100% identity with the following protein names: -diaphanous 2 isoform 156 (human) -diaphanous protein homolog 2 (diaphanous related formin 2, DRF2) (human) -DIA-156 protein (human) The sequence has 90% identity with: -DIA3 (mouse) -DRF2, mDIA3 (mouse) -DIA (mouse) The sequence gas 68% identity with: -diaphanous homolog 2 of chicken Therefore the antibody should recognise diaphanous 2 isoform 156 (human), diaphanous protein homolog 2, (diaphanous related formin 2, DRF2), DIA-156 protein (human) and as the homology with mouse is quite high it may cross react with mouse. I hope this information is useful and thank you again for your input on this matter,

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