Key features and details
- Rabbit polyclonal to DIAPH2/DIA
- Suitable for: WB
- Reacts with: Mouse, Rat, Rabbit, Human
- Isotype: IgG
Product nameAnti-DIAPH2/DIA antibody
See all DIAPH2/DIA primary antibodies
DescriptionRabbit polyclonal to DIAPH2/DIA
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Human
Synthetic peptide corresponding to Mouse DIAPH2/DIA (C terminal) conjugated to keyhole limpet haemocyanin.
- A431, HeLa, Mouse brain tissue, C2C12 cells, Rat PC12 and Rabbit spleen fibroblast lysates.
This product was previously labelled as DIAPH2. Do not aliquot.
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Storage instructionsShipped at 4°C. Store at -20°C. Please see notes section. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab119702 was affinity purified using a DIAPH2/DIA (C terminal region) peptide (without carrier).
Our Abpromise guarantee covers the use of ab119702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 126 kDa).|
FunctionCould be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.
Tissue specificityExpressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes.
Involvement in diseaseDefects in DIAPH2 are the cause of premature ovarian failure type 2A (POF2A) [MIM:300511]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
Sequence similaritiesBelongs to the formin homology family. Diaphanous subfamily.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
Developmental stageExpressed from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles.
DomainDRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
Cellular localizationCytoplasm > cytosol. Early endosome. Isoform 3 is cytosolic but when coexpressed with RHOD, the 2 proteins colocalize to early endosomes.
- Information by UniProt
- Dia 2 antibody
- DIA antibody
- Dia drome antibody
ab119702 has not yet been referenced specifically in any publications.