Key features and details
- Rabbit polyclonal to DIAPH2/DIA
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DIAPH2/DIA antibody
See all DIAPH2/DIA primary antibodies
DescriptionRabbit polyclonal to DIAPH2/DIA
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Human DIAPH2/DIA aa 794-997.
KMLQPRLSSILFKLTFEEHINNIKPSIIAVTLACEELKKSESFNRLLELV LLVGNYMNSGSRNAQSLGFKINFLCKIRDTKSADQKTTLLHFIADICEEK YRDILKFPEELEHVESASKVSAQILKSNLASMEQQIVHLERDIKKFPQAE NQHDKFVEKMTSFTKTAREQYEKLSTMHNNMMKLYENLGEYFIFDSKTVS IEE
Database link: BC117414
- Mouse brain tissue lysate; Human fetal testis tissue.
This product was previously labelled as DIAPH2
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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FormLyophilized:Reconstitute in 200ul Sterile Distilled Water.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab201205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/1000. Predicted molecular weight: 126 kDa.|
|IHC-P||1/100 - 1/500.|
FunctionCould be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.
Tissue specificityExpressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes.
Involvement in diseaseDefects in DIAPH2 are the cause of premature ovarian failure type 2A (POF2A) [MIM:300511]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
Sequence similaritiesBelongs to the formin homology family. Diaphanous subfamily.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
Developmental stageExpressed from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles.
DomainDRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
Cellular localizationCytoplasm > cytosol. Early endosome. Isoform 3 is cytosolic but when coexpressed with RHOD, the 2 proteins colocalize to early endosomes.
- Information by UniProt
- Dia 2 antibody
- DIA antibody
- Dia drome antibody
ab201205 has not yet been referenced specifically in any publications.