Overview

  • Product name

  • Description

    Rabbit polyclonal to DIAPH2/DIA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DIAPH2/DIA aa 870-899 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: O60879

  • Positive control

    • WB: HEK-293 and MDS-MB-345 cell lysates. IHC-P: Human colon tissue. Flow Cyt: CEM cells.
  • General notes

     This product was previously labelled as DIAPH2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab230804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 126 kDa.
Flow Cyt 1/10 - 1/50.

Target

  • Function

    Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.
  • Tissue specificity

    Expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes.
  • Involvement in disease

    Defects in DIAPH2 are the cause of premature ovarian failure type 2A (POF2A) [MIM:300511]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similarities

    Belongs to the formin homology family. Diaphanous subfamily.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • Developmental stage

    Expressed from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles.
  • Domain

    DRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
  • Cellular localization

    Cytoplasm > cytosol. Early endosome. Isoform 3 is cytosolic but when coexpressed with RHOD, the 2 proteins colocalize to early endosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dia 2 antibody
    • DIA antibody
    • Dia drome antibody
    • Dia2 antibody
    • Diap 2 antibody
    • Diap2 antibody
    • DIAP2_HUMAN antibody
    • DIAPH 2 antibody
    • Diaph2 antibody
    • Diaphanous 2 antibody
    • Diaphanous homolog 2 (Drosophila) antibody
    • Diaphanous homolog 2 antibody
    • Diaphanous related formin 2 antibody
    • Diaphanous-related formin-2 antibody
    • Diaphanous2 antibody
    • Diaphorase 2 antibody
    • Diaphorase2 antibody
    • DRF 2 antibody
    • DRF2 antibody
    • FLJ11167 antibody
    • OTTHUMP00000024270 antibody
    • OTTHUMP00000024271 antibody
    • OTTHUMP00000062171 antibody
    • POF 2 antibody
    • POF antibody
    • POF2 antibody
    • Protein diaphanous homolog 2 antibody
    see all

Images

  • All lanes : Anti-DIAPH2/DIA antibody (ab230804) at 1/1000 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : MDA-MB-435 cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 126 kDa

  • Formalin-fixed, paraffin-embedded human colon tissue stained for DIAPH2/DIA using ab230804 at 1/75 dilution in immunohistochemical analysis.

  • Flow cytometric analysis of CEM cells labeling DIAPH2/DIA with ab230804 at 1/10 dilution (green) compared with a negative control (blue).

    FITC-conjugated goat-anti-rabbit secondary antibodies were used.

References

ab230804 has not yet been referenced specifically in any publications.

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