Key features and details
- Rabbit polyclonal to DIAPH2/DIA
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DIAPH2/DIA antibody
See all DIAPH2/DIA primary antibodies
DescriptionRabbit polyclonal to DIAPH2/DIA
Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human DIAPH2/DIA aa 870-899 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: O60879
- WB: HEK-293 and MDS-MB-345 cell lysates. IHC-P: Human colon tissue. Flow Cyt: CEM cells.
This product was previously labelled as DIAPH2
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab230804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 126 kDa.|
|Flow Cyt||1/10 - 1/50.|
FunctionCould be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.
Tissue specificityExpressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes.
Involvement in diseaseDefects in DIAPH2 are the cause of premature ovarian failure type 2A (POF2A) [MIM:300511]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
Sequence similaritiesBelongs to the formin homology family. Diaphanous subfamily.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
Developmental stageExpressed from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles.
DomainDRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
Cellular localizationCytoplasm > cytosol. Early endosome. Isoform 3 is cytosolic but when coexpressed with RHOD, the 2 proteins colocalize to early endosomes.
- Information by UniProt
- Dia 2 antibody
- DIA antibody
- Dia drome antibody
All lanes : Anti-DIAPH2/DIA antibody (ab230804) at 1/1000 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : MDA-MB-435 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 126 kDa
Formalin-fixed, paraffin-embedded human colon tissue stained for DIAPH2/DIA using ab230804 at 1/75 dilution in immunohistochemical analysis.
Flow cytometric analysis of CEM cells labeling DIAPH2/DIA with ab230804 at 1/10 dilution (green) compared with a negative control (blue).
FITC-conjugated goat-anti-rabbit secondary antibodies were used.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230804 has not yet been referenced specifically in any publications.