Recombinant Anti-DIAPH2/DIA antibody [EPR13158] - BSA and Azide free (ab250391)
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Overview
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Product name
Anti-DIAPH2/DIA antibody [EPR13158] - BSA and Azide free
See all DIAPH2/DIA primary antibodies -
Description
Rabbit monoclonal [EPR13158] to DIAPH2/DIA - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human DIAPH2/DIA aa 900-1000. The exact sequence is proprietary.
Database link: O60879 -
General notes
ab250391 is the carrier-free version of ab181165 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab250391 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as DIAPH2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13158 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab250391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 126 kDa). |
Target
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Function
Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton. -
Tissue specificity
Expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes. -
Involvement in disease
Defects in DIAPH2 are the cause of premature ovarian failure type 2A (POF2A) [MIM:300511]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. -
Sequence similarities
Belongs to the formin homology family. Diaphanous subfamily.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain. -
Developmental stage
Expressed from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles. -
Domain
DRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction. -
Cellular localization
Cytoplasm > cytosol. Early endosome. Isoform 3 is cytosolic but when coexpressed with RHOD, the 2 proteins colocalize to early endosomes. - Information by UniProt
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Database links
- Entrez Gene: 1730 Human
- Entrez Gene: 54004 Mouse
- Entrez Gene: 317189 Rat
- Omim: 300108 Human
- SwissProt: O60879 Human
- SwissProt: O70566 Mouse
- Unigene: 226483 Human
- Unigene: 10763 Mouse
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Alternative names
- Dia 2 antibody
- DIA antibody
- Dia drome antibody
see all
Datasheets and documents
References
ab250391 has not yet been referenced specifically in any publications.
