Recombinant Anti-DIAPH2/DIA antibody [EPR13158] - BSA and Azide free (ab250391)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13158] to DIAPH2/DIA - BSA and Azide free
- Suitable for: WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-DIAPH2/DIA antibody [EPR13158] - BSA and Azide free
See all DIAPH2/DIA primary antibodies -
Description
Rabbit monoclonal [EPR13158] to DIAPH2/DIA - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250391 is the carrier-free version of ab181165 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab250391 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as DIAPH2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13158 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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All applications |
Mouse
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Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 126 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 126 kDa). |
Target
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Function
Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton. -
Tissue specificity
Expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes. -
Involvement in disease
Defects in DIAPH2 are the cause of premature ovarian failure type 2A (POF2A) [MIM:300511]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. -
Sequence similarities
Belongs to the formin homology family. Diaphanous subfamily.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain. -
Developmental stage
Expressed from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles. -
Domain
DRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction. -
Cellular localization
Cytoplasm > cytosol. Early endosome. Isoform 3 is cytosolic but when coexpressed with RHOD, the 2 proteins colocalize to early endosomes. - Information by UniProt
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Database links
- Entrez Gene: 1730 Human
- Entrez Gene: 54004 Mouse
- Entrez Gene: 317189 Rat
- Omim: 300108 Human
- SwissProt: O60879 Human
- SwissProt: O70566 Mouse
- Unigene: 226483 Human
- Unigene: 10763 Mouse
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Alternative names
- Dia 2 antibody
- DIA antibody
- Dia drome antibody
see all
Images
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All lanes : Anti-DIAPH2/DIA antibody [EPR13158] (ab181165) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HCT-116 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 126 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?This data was developed using ab181165, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab250391 has not yet been referenced specifically in any publications.