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RecombinantRabMAb

Recombinant Anti-DIAPH2/DIA antibody [EPR13158] - BSA and Azide free (ab250391)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-DIAPH2/DIA antibody [EPR13158] - BSA and Azide free (ab250391)
  • Anti-DIAPH2/DIA antibody [EPR13158] - BSA and Azide free (ab250391)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13158] to DIAPH2/DIA - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Rat, Human

You may also be interested in

Conjugation
Product image
HRP Conjugation Kit - Lightning-Link® (ab102890)

View more associated products

Overview

  • Product name

    Anti-DIAPH2/DIA antibody [EPR13158] - BSA and Azide free
    See all DIAPH2/DIA primary antibodies
  • Description

    Rabbit monoclonal [EPR13158] to DIAPH2/DIA - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250391 is the carrier-free version of ab181165 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab250391 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as DIAPH2

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13158
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Associated products

  • Alternative Versions

    • Anti-DIAPH2/DIA antibody [EPR13158] (ab181165)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 126 kDa).
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 126 kDa).

Target

  • Function

    Could be involved in oogenesis. Involved in the regulation of endosome dynamics. Implicated in a novel signal transduction pathway, in which isoform 3 and CSK are sequentially activated by RHOD to regulate the motility of early endosomes through interactions with the actin cytoskeleton.
  • Tissue specificity

    Expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes.
  • Involvement in disease

    Defects in DIAPH2 are the cause of premature ovarian failure type 2A (POF2A) [MIM:300511]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
  • Sequence similarities

    Belongs to the formin homology family. Diaphanous subfamily.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • Developmental stage

    Expressed from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles.
  • Domain

    DRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
  • Cellular localization

    Cytoplasm > cytosol. Early endosome. Isoform 3 is cytosolic but when coexpressed with RHOD, the 2 proteins colocalize to early endosomes.
  • Target information above from: UniProt accession O60879 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1730 Human
    • Entrez Gene: 54004 Mouse
    • Entrez Gene: 317189 Rat
    • Omim: 300108 Human
    • SwissProt: O60879 Human
    • SwissProt: O70566 Mouse
    • Unigene: 226483 Human
    • Unigene: 10763 Mouse
    • Alternative names

      • Dia 2 antibody
      • DIA antibody
      • Dia drome antibody
      • Dia2 antibody
      • Diap 2 antibody
      • Diap2 antibody
      • DIAP2_HUMAN antibody
      • DIAPH 2 antibody
      • Diaph2 antibody
      • Diaphanous 2 antibody
      • Diaphanous homolog 2 (Drosophila) antibody
      • Diaphanous homolog 2 antibody
      • Diaphanous related formin 2 antibody
      • Diaphanous-related formin-2 antibody
      • Diaphanous2 antibody
      • Diaphorase 2 antibody
      • Diaphorase2 antibody
      • DRF 2 antibody
      • DRF2 antibody
      • FLJ11167 antibody
      • OTTHUMP00000024270 antibody
      • OTTHUMP00000024271 antibody
      • OTTHUMP00000062171 antibody
      • POF 2 antibody
      • POF antibody
      • POF2 antibody
      • Protein diaphanous homolog 2 antibody
      see all

    Images

    • Western blot - Anti-DIAPH2/DIA antibody [EPR13158] - BSA and Azide free (ab250391)
      Western blot - Anti-DIAPH2/DIA antibody [EPR13158] - BSA and Azide free (ab250391)
      All lanes : Anti-DIAPH2/DIA antibody [EPR13158] (ab181165) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HCT-116 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 126 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?



      This data was developed using ab181165, the same antibody clone in a different buffer formulation.

       

    • Anti-DIAPH2/DIA antibody [EPR13158] - BSA and Azide free (ab250391)
      Anti-DIAPH2/DIA antibody [EPR13158] - BSA and Azide free (ab250391)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab250391? Please let us know so that we can cite the reference in this datasheet.

    ab250391 has not yet been referenced specifically in any publications.

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