Overview

  • Product name

  • Description

    Rabbit Polyclonal to DIAPH3
  • Host species

    Rabbit
  • Specificity

    ab189373 is reactive against recombinant protein, detects immunogen.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Positive control

    • Human adrenal tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.79% Tris glycine, 10% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 137 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics.
  • Sequence similarities

    Belongs to the formin homology family. Diaphanous subfamily.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • Domain

    DRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
  • Information by UniProt
  • Database links

  • Alternative names

    • AN antibody
    • AUNA1 antibody
    • Dia2 antibody
    • diap3 antibody
    • DIAP3_HUMAN antibody
    • DIAPH3 antibody
    • Diaphanous homolog 3 (Drosophila) antibody
    • Diaphanous homolog 3 antibody
    • Diaphanous related formin 3 antibody
    • Diaphanous, Drosophila, homolog of, 3 antibody
    • Diaphanous-related formin-3 antibody
    • DKFZp434C0931 antibody
    • DKFZp686A13178 antibody
    • DRF3 antibody
    • FLJ34705 antibody
    • mDia2 antibody
    • NSDAN antibody
    • OTTHUMP00000018480 antibody
    • Protein diaphanous homolog 3 antibody
    • RP11-26P21.1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human adrenal tissue, labeling DIAPH3 using ab189373 at 10 µg/ml.

References

ab189373 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189373.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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