Overview

  • Product name

  • Description

    Rabbit polyclonal to DIAPH3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human DIAPH3 aa 1143-1193. The exact sequence is proprietary.
    Database link: Q9NSV4

  • Positive control

    • WB: HeLa and HEK-239T whole cell lysate. IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245660 was affinity purified using an epitope specific to DIAPH3 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 137 kDa.

Target

  • Function

    Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics.
  • Sequence similarities

    Belongs to the formin homology family. Diaphanous subfamily.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • Domain

    DRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
  • Information by UniProt
  • Database links

  • Alternative names

    • AN antibody
    • AUNA1 antibody
    • Dia2 antibody
    • diap3 antibody
    • DIAP3_HUMAN antibody
    • DIAPH3 antibody
    • Diaphanous homolog 3 (Drosophila) antibody
    • Diaphanous homolog 3 antibody
    • Diaphanous related formin 3 antibody
    • Diaphanous, Drosophila, homolog of, 3 antibody
    • Diaphanous-related formin-3 antibody
    • DKFZp434C0931 antibody
    • DKFZp686A13178 antibody
    • DRF3 antibody
    • FLJ34705 antibody
    • mDia2 antibody
    • NSDAN antibody
    • OTTHUMP00000018480 antibody
    • Protein diaphanous homolog 3 antibody
    • RP11-26P21.1 antibody
    see all

Images

  • All lanes : Anti-DIAPH3 antibody (ab245660) at 0.1 µg

    Lane 1 : HeLa (Human bone marrow erythroleukemia cell line) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 137 kDa


    Exposure time: 10 seconds


    Prepared using NETN lysis buffer.

  • DIAPH3 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

    ab245660 used for IP at 6 µg per reaction. For WB 1 µg/ml.

    Lane 1: ab245660 IP in HeLa whole cell lysate.
    Lane 2: Control IgG in HeLa whole cell lysate.

    Chemiluminescence detection: 3 seconds.

References

ab245660 has not yet been referenced specifically in any publications.

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