Key features and details
- Rabbit polyclonal to DIAPH3
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DIAPH3 antibody
See all DIAPH3 primary antibodies
DescriptionRabbit polyclonal to DIAPH3
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human DIAPH3 aa 1143-1193. The exact sequence is proprietary.
Database link: Q9NSV4
- WB: HeLa and HEK-239T whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245660 was affinity purified using an epitope specific to DIAPH3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 137 kDa.|
FunctionBinds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics.
Sequence similaritiesBelongs to the formin homology family. Diaphanous subfamily.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
DomainDRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
- Information by UniProt
- AN antibody
- AUNA1 antibody
- Dia2 antibody
All lanes : Anti-DIAPH3 antibody (ab245660) at 0.1 µg
Lane 1 : HeLa (Human bone marrow erythroleukemia cell line) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 137 kDa
Exposure time: 10 seconds
Prepared using NETN lysis buffer.
DIAPH3 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245660 used for IP at 6 µg per reaction. For WB 1 µg/ml.
Lane 1: ab245660 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Chemiluminescence detection: 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245660 has not yet been referenced specifically in any publications.