Key features and details
- Rabbit Polyclonal to DIAPH3 (phospho T1061 + T1061)
- Suitable for: WB
- Reacts with: Mouse, Rat, Chicken, Human, Frog
- Isotype: IgG
Product nameAnti-DIAPH3 (phospho T1061 + T1061) antibody
See all DIAPH3 primary antibodies
DescriptionRabbit Polyclonal to DIAPH3 (phospho T1061 + T1061)
SpecificityThe immunogen sequence for ab202281 has low homology to other formins.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human, Frog
Synthetic peptide corresponding to Mouse DIAPH3 (phospho T1061) conjugated to Keyhole Limpet Haemocyanin (KLH). A phospho specific synthetic peptide corresponding to residues surrounding threonine 1061. This peptide sequence is identical in rat and has 2 amino acid differences to Human mDia2.
Database link: Q9Z207
- Monkey COS7 cells transfected with recombinant DIAPH3, untreated or treated with calyculin A.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab202281 was affinity purified using DIAPH3 (Thr-1061) peptide (without carrier).
Our Abpromise guarantee covers the use of ab202281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 134 kDa.|
FunctionBinds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics.
Sequence similaritiesBelongs to the formin homology family. Diaphanous subfamily.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
DomainDRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
- Information by UniProt
- AN antibody
- AUNA1 antibody
- Dia2 antibody
All lanes : Anti-DIAPH3 (phospho T1061 + T1061) antibody (ab202281) at 1/1000 dilution
Lane 1 : monkey COS7 cells transfected with recombinant DIAPH3
Lane 2 : monkey COS7 cells transfected with recombinant DIAPH3 treated with Calyculin A and alkaline phosphatase
Lane 3 : monkey COS7 cells transfected with recombinant DIAPH3 treated with calyculin A
Lane 4 : monkey COS7 cells transfected with recombinant DIAPH3 (T1061A)
Predicted band size: 134 kDa
The lower blot was probed with anti-DDDDK antibody.
ab202281 has not yet been referenced specifically in any publications.