Product nameAnti-DIAPH3 (phospho T1061 + T1061) antibody
See all DIAPH3 primary antibodies
DescriptionRabbit Polyclonal to DIAPH3 (phospho T1061 + T1061)
SpecificityThe immunogen sequence for ab202281 has low homology to other formins.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human, Frog
Synthetic peptide corresponding to Mouse DIAPH3 (phospho T1061) conjugated to Keyhole Limpet Haemocyanin (KLH). A phospho specific synthetic peptide corresponding to residues surrounding threonine 1061. This peptide sequence is identical in rat and has 2 amino acid differences to Human mDia2.
Database link: Q9Z207
- Monkey COS7 cells transfected with recombinant DIAPH3, untreated or treated with calyculin A.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab202281 was affinity purified using DIAPH3 (Thr-1061) peptide (without carrier).
Our Abpromise guarantee covers the use of ab202281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 134 kDa.|
FunctionBinds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics.
Sequence similaritiesBelongs to the formin homology family. Diaphanous subfamily.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
DomainDRFs are regulated by intramolecular GBD-DAD binding where Rho-GTP activates the DRFs by disrupting the GBD-DAD interaction.
- Information by UniProt
- AN antibody
- AUNA1 antibody
- Dia2 antibody
All lanes : Anti-DIAPH3 (phospho T1061 + T1061) antibody (ab202281) at 1/1000 dilution
Lane 1 : monkey COS7 cells transfected with recombinant DIAPH3
Lane 2 : monkey COS7 cells transfected with recombinant DIAPH3 treated with Calyculin A and alkaline phosphatase
Lane 3 : monkey COS7 cells transfected with recombinant DIAPH3 treated with calyculin A
Lane 4 : monkey COS7 cells transfected with recombinant DIAPH3 (T1061A)
Predicted band size: 134 kDa
The lower blot was probed with anti-DDDDK antibody.
ab202281 has not yet been referenced specifically in any publications.