The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
IP: Use at a concentration of 1 - 2 µg/ml lysate.
WB: 1/100 - 1/500. Predicted molecular weight: 10 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.
Isoform 1 is ubiquitous, with a moderate expression level. Isoform 2 is ubiquitous with high level in liver and adipose tissue. Isoform 3 is ubiquitous with strong expression in adipose tissue and heart.
Belongs to the ACBP family. Contains 1 ACB (acyl-CoA-binding) domain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Diazepam Binding Inhibitor antibody [27C9] (ab16806)This image is courtesy of an abreview submitted by Aamir Ahmed, University College London, United Kingdom.
IHC-P image of Diazepam Binding Inhibitor antibody (ab16806) on Lung cancer sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 3% H2O2 solution for 10 minutes at 22°C.
All lanes : Anti-Diazepam Binding Inhibitor antibody [27C9] (ab16806) at 1/250 dilution
Lane 1 : HepG2 Cell lysate Lanes 2-3 : Precipitated from HepG2 Cell lysate at 200 µg Lane 4 : Precipitated from PBS
Western blot - Anti-Diazepam Binding Inhibitor antibody [27C9] (ab16806)
ab16806 at a 1/500 dilution staining approx. 10kDa Diazepam binding inhibitor in HepG2 cell lysate by Western blot (ECL). ab16806 at a 1/500 dilution staining approx. 10kDa Diazepam binding inhibitor in HepG2 cell lysate by Western blot (ECL).
Harris FT et al. Acyl-coenzyme A-binding protein regulates Beta-oxidation required for growth and survival of non-small cell lung cancer. Cancer Prev Res (Phila)7:748-57 (2014).
Read more (PubMed: 24819876) »