Anti-Dibromotyrosine antibody [9F12] (ab240911)

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Overview

  • Product name

    Anti-Dibromotyrosine antibody [9F12]
    See all Dibromotyrosine primary antibodies

  • Description

    Mouse monoclonal [9F12] to Dibromotyrosine

  • Host species

    Mouse

  • Specificity

    Does not cross-react with Nitrotyrosine.

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Chemical/ Small Molecule corresponding to Dibromotyrosine conjugated to keyhole limpet haemocyanin.

  • Positive control

    • WB: Dibromotyrosine-BSA; HeLa cells treated with hydrogen peroxide.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.4
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from TCS.

  • Clonality

    Monoclonal

  • Clone number

    9F12

  • Isotype

    IgG1

Applications

Our Abpromise guarantee covers the use of ab240911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.

Target

  • Alternative names

    • 3,5-Dibromo-tyrosine antibody
    • 3,5-dibromotyrosine antibody
    • Dibromo-tyrosine antibody
    • DiBrY antibody

Images

  • Western blot - Anti-Dibromotyrosine antibody [9F12] (ab240911)
    Western blot - Anti-Dibromotyrosine antibody [9F12] (ab240911)
    All lanes : 2 hours at RT at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : Hydrogen peroxidase treated HeLa cell lysate

    Lysates/proteins at 12 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG-HRP for 60 minutes at RT at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 5 minutes


    Block: 5% Skimmed milk in TBST.

  • Western blot - Anti-Dibromotyrosine antibody [9F12] (ab240911)
    Western blot - Anti-Dibromotyrosine antibody [9F12] (ab240911)
    All lanes : Anti-Dibromotyrosine antibody [9F12] (ab240911) at 1/1000 dilution (2 hours at RT)

    Lane 1 : BSA
    Lane 2 : Nitrosylated-BSA
    Lane 3 : Dibromotyrosine-BSA

    Lysates/proteins at 1 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Mouse IgG-HRP for 60 minutes at RT. at 1/2000 dilution
    Lane 3 : Goat Anti-Mouse IgG-HRP for 60 minutes at RT at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 5 minutes


    Block: 5% Skimmed milk in TBST.

References

ab240911 has not yet been referenced specifically in any publications.

Publishing research using ab240911? Please let us know so that we can cite the reference in this datasheet.

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