Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)

The peptide immunogen was xxxxxxxx which corresponds to xxxxxxxx of the immature unprocessed sequence.

I would therefore suggest you use the following blocking peptide: Dicer peptide (ab24556)

I am very pleased to hear you would like to test ab14601 in rat. This code will give you $379 off your next order before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

Acording to UniProt, http://www.uniprot.org/uniprot/Q9UPY3, there are 2 isoforms of the Human Endoribonuclease Dicer (Gene name = Dicer1).
The isoform 2, also known as t-Dicer, differs from the canonical sequence as follows: 1789-1922: LRRSEEDEEK...KANQPQVPNS → KSFLQMYPVP...TTGRSESLWK.
The anti-Dicer ab14601, targeting Dicer specifically in the region between amino acids 1239 and 1255 of the PAZ domain, can therefore bind to both isoforms described on http://www.uniprot.org/uniprot/Q9UPY3.

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from the last 2 vials of this antibody.

The details you have kindly provided will provide us with vital information for our monitoring of product quality

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results from the last 2 vials have not been successful.

I traced the order number for the two vials that did not work:

Order ##### Lot number GR97114-5
Order ##### Lot number GR97114-8

These were indeed from the same original lot number (GR97114). Unfortunately, I checked our system and I am very sorry to confirm we regrettably do not have any different lots in stock at the moment.

I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch. Regrettably, I can suggest you have received bad vials on this occasion. Of course your enquiry will remain on our complaint records for continuing monitoring.

Therefore, I would be pleased to provide two replacement vials from the same lot, which will still be covered by our guarantee. Alternatively, I fully understand your concerns and if you prefer a credit note in this case, I will be pleased to arrange this for you.

I am sorry I have still not managed to trace your current order. I have alerted our customer service team to your request and asked them to let me know if they see the order coming through. However, we do have many orders coming through. Please let me know how you would like to proceed with this order, if you want to go ahead or cancel.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Thank you for contacting Abcam.
The peptide used to generate this antibody was located between amino acids *****of the human form of the protein (SwissProt: Q9UPY3 Human).
Please let me know if there is anything else I can help you with, Free RabMab with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Thank you very much for your call today and for letting us know about the trouble with this antibody.

Here areour suggestions for transferring high molecular weight proteins (>100 kDa):

For large proteins, transfer out of the gel may be very slow, just as they run slowly within the gel during separation. If blotting a large protein, be sure to run your samples in a low-concentration gel, 8% or less. These will be very fragile, so handle carefully.
Large proteins will tend to precipitate in the gel, hindering transfer. Adding SDS to a final concentration of 0.1% in the transfer buffer will discourage this. Methanol tends to remove SDS from proteins, so reducing the methanol percentage to 10% or less will also guard against precipitation.
Lowering methanol in the transfer buffer also promotes swelling of the gel, allowing large proteins to transfer more easily.
Methanol is only necessary if using nitrocellulose. If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich.
Choose wet transfer overnight at4°C instead of semi-dry transfer.



Generally for an overnight transfer, manufacturers recommend 10-30V depending on the equipment that you have. If you'd like to send me the name of the transfer equipment you use, I'll be happy to look up the specific suggestion (I've attached the BioRad guide, see page 24 if you're using one of their transfer boxes).

I hope that this information will be useful, but we do fully guarantee this antibody to work in your assay, so if the problem persists then I would be happy to send a replacement or issue a credit or refund. Please keep me updated about the results, and let me know if there is anything else that we can do for you.

Have a nice day!