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  1. Link

    dicer-antibody-13d6-chip-grade-ab14601.pdf

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Validated using a knockout cell line

Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)

  • Datasheet
  • SDS
Reviews (16)Q&A (32)References (138)

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Western blot - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)
  • Western blot - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)
  • Flow Cytometry - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)
  • Western blot - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)
  • ChIP - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)
  • Western blot - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)

Key features and details

  • Mouse monoclonal [13D6] to Dicer - ChIP Grade
  • Suitable for: WB, ChIP, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-Dicer antibody [13D6] - ChIP Grade
    See all Dicer primary antibodies
  • Description

    Mouse monoclonal [13D6] to Dicer - ChIP Grade
  • Host species

    Mouse
  • Specificity

    We do not guarantee its use in WB with mouse lysates.
  • Tested applications

    Suitable for: WB, ChIP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Does not react with: Caenorhabditis elegans
  • Immunogen

    Synthetic peptide corresponding to Human Dicer.

  • Positive control

    • WB: 10ng of recombinant dicer (using ECL or ECL Plus) in under one minute of exposure to film. ICC: Human Fibroblasts
  • General notes

    To our knowledge the localization of Dicer remains to be fully determined and it appears that its localization can be nuclear and/or cytoplasmic. Please see reference Meltzer, 2005 for a reference to a cytoplasmic localisation (figure 1 of paper), as supported with the staining seen in the image on this datasheet.
    Please note this antibody gave negative results in WB with mouse fibroblast cell line lysates. We do not guarantee its use in WB with mouse lysates.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: HEPES buffered saline
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Clonality

    Monoclonal
  • Clone number

    13D6
  • Isotype

    IgG2a
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Dicer
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

Associated products

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab14601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (9)
1/100 - 1/2000.

Please note this antibody gave negative results in WB with mouse fibroblast cell line lysates. We do not guarantee its use in WB with mouse lysates.

ChIP
Use at an assay dependent concentration.
Flow Cyt
Use 0.1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Notes
WB
1/100 - 1/2000.

Please note this antibody gave negative results in WB with mouse fibroblast cell line lysates. We do not guarantee its use in WB with mouse lysates.

ChIP
Use at an assay dependent concentration.
Flow Cyt
Use 0.1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
  • Involvement in disease

    Pleuropulmonary blastoma
    Goiter multinodular 1, with or without Sertoli-Leydig cell tumors
    Rhabdomyosarcoma, embryonal, 2
    DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960).
  • Sequence similarities

    Belongs to the helicase family. Dicer subfamily.
    Contains 1 Dicer dsRNA-binding fold domain.
    Contains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 PAZ domain.
    Contains 2 RNase III domains.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q9UPY3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23405 Human
    • Entrez Gene: 192119 Mouse
    • Omim: 606241 Human
    • SwissProt: Q9UPY3 Human
    • SwissProt: Q8R418 Mouse
    • Unigene: 738957 Human
    • Unigene: 87889 Human
    • Unigene: 21135 Mouse
    • Alternative names

      • DCR antibody
      • DCR1 antibody
      • Dicer 1 ribonuclease III antibody
      • Dicer 1 ribonuclease type III antibody
      • Dicer antibody
      • DICER_HUMAN antibody
      • DICER1 antibody
      • Double-strand-specific ribonuclease antibody
      • Endoribonuclease Dicer antibody
      • Helicase MOI antibody
      • Helicase with RNase motif antibody
      • HERNA antibody
      • KIAA0928 antibody
      • MNG1 antibody
      • RMSE2 antibody
      see all

    Images

    • Western blot - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)
      Western blot - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Dicer knockout HAP1 cell lysate (20 µg)

      Lanes 1 and 2: Merged signal (red and green). Green - ab14601 observed at 240 kDa. Red - loading control, ab181602, observed at 37 kDa.


      ab14601 was shown to specifically react with Dicer in wild-type HAP1 cells. No band was observed when Dicer knockout samples were used. Wild-type and Dicer knockout samples were subjected to SDS-PAGE. ab14601 and ab181602 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

    • Western blot - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)
      Western blot - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)
      All lanes : Anti-Dicer antibody [13D6] - ChIP Grade (ab14601) at 1/2000 dilution (incubated overnight at 4°C.)

      Lane 1 : NIH 3T3
      Lane 2 : A549
      Lane 3 : HepG2

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Mouse Green at 1/10000 dilution


      This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab14601 overnight at 4°C. Antibody binding was detected using IR-labelled goat anti-mouse Ab at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    • Flow Cytometry - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)
      Flow Cytometry - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)
      Overlay histogram showing HEK293 cells stained with ab14601 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14601, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Western blot - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)
      Western blot - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)
      HepG2 cell lysate at 20 µg
      Developed using the ECL technique.

      Performed under reducing conditions.

      Additional bands at: 100 kDa (possible non-specific binding), 60 kDa (possible non-specific binding)

    • ChIP - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)
      ChIP - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)This image is courtesy of Elliot Epner MD PhD, Oregon Health and Science University, Portland

      ChIP analysis of Dicer protein binding at chicken ß-globin regulatory elements. Chromatin from human erythroleukemia (K562) cells containing chicken chromosomes with normal (FRK2) or mutant (HS1-K) ß-globin loci and chicken DT40 cells was immunprecipitated with antibodies to Dicer (ab14601). PCR analysis of immunoprecipitated chromatin was carried out using primers to the chicken ß-globin regulatory elements. Mouse IgG served as control. 10% of input DNA was utilized.

    • Western blot - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)
      Western blot - Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)This image is courtesy of Jing Huang and Elliot Epner Oregon Health and Science University
      All lanes : Anti-Dicer antibody [13D6] - ChIP Grade (ab14601) at 2.5 µg/ml

      Lane 1 : human B cell lymphoma lysate
      Lane 2 : human B cell lymphoma lysate infected with control shRNA retrovirus
      Lane 3 : human B cell lymphoma lysate infected with Dicer knockdown shRNA virus

      Lysates/proteins at 2.5 µg/ml per lane.

      Observed band size: 225 kDa why is the actual band size different from the predicted?

    Protocols

    • ChIP protocols
    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (138)

    Publishing research using ab14601? Please let us know so that we can cite the reference in this datasheet.

    ab14601 has been referenced in 138 publications.

    • Shirazi S  et al. The importance of cellular and exosomal miRNAs in mesenchymal stem cell osteoblastic differentiation. Sci Rep 11:5953 (2021). PubMed: 33723364
    • Zhang Q  et al. FoxP3-miR-150-5p/3p suppresses ovarian tumorigenesis via an IGF1R/IRS1 pathway feedback loop. Cell Death Dis 12:275 (2021). PubMed: 33723215
    • Wu J  et al. Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells. Cell Res 31:998-1010 (2021). PubMed: 34158624
    • Brandão BB  et al. Dynamic changes in DICER levels in adipose tissue control metabolic adaptations to exercise. Proc Natl Acad Sci U S A 117:23932-23941 (2020). PubMed: 32900951
    • Paulsson JO  et al. GABPA-dependent down-regulation of DICER1 in follicular thyroid tumours. Endocr Relat Cancer 27:295-308 (2020). PubMed: 32163919
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 48 Abreviews or Q&A

    Western blot abreview for Anti-Dicer antibody [13D6] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (thyroid cancer cells Cal62 and TPC1)
    Gel Running Conditions
    Reduced Denaturing (7%)
    Loading amount
    50 µg
    Treatment
    DICER1 silencing
    Specification
    thyroid cancer cells Cal62 and TPC1
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 08 2022

    Western blot abreview for Anti-Dicer antibody [13D6] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Hamster Cell lysate - whole cell (VeroE6 (Kidney from African green monkey), BHK21 ()
    Gel Running Conditions
    Reduced Denaturing (4-12% NuPage gel, MES buffer)
    Loading amount
    25000 cells
    Specification
    VeroE6 (Kidney from African green monkey), BHK21 (
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 11 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Dicer [13D6] antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Sample
    Human Cell (Hela)
    Specification
    Hela
    Permeabilization
    Yes - 0.05% tritonx-100
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. santosh chauhan

    Verified customer

    Submitted Apr 14 2014

    Western blot abreview for Anti-Dicer antibody [13D6] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Mammary Tumor)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    60 µg
    Specification
    Mammary Tumor
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 31 2013

    Western blot

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    60 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Rat Tissue lysate - whole (Mammary Tumor)
    Specification
    Mammary Tumor
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 30 2013

    Question

    Inquiry: We are looking to perform a blocking experiment with this antibody. Which of your Dicer peptides is suitable for this experiment?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 22 2013

    Answer

    The peptide immunogen was xxxxxxxx which corresponds to xxxxxxxx of the immature unprocessed sequence.

    I would therefore suggest you use the following blocking peptide: Dicer peptide (ab24556)

    Read More

    Elisa Thomas

    Abcam Scientific Support

    Answered on Jul 22 2013

    Question

    I would like to test this antibody in rat, an untested species.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 01 2013

    Answer

    I am very pleased to hear you would like to test ab14601 in rat. This code will give you $379 off your next order before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

    Read More

    Abcam Scientific Support

    Answered on Feb 01 2013

    Question

    I do not know which Dicer isoform contains (1239 - 1255 of Human Dicer, PAZ domain), but I assume this is Dicer-1 isoform, because:
    1) You say it is Human Dicer (PAZ domain), my understanding is in human only Dicer1 exists. Is that right?
    2) In the data sheet, it says that the alternative name is Dicer1 antibody.
    Do you agree that it is Dicer1 that this antibody attached to?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 20 2012

    Answer


    Acording to UniProt, http://www.uniprot.org/uniprot/Q9UPY3, there are 2 isoforms of the Human Endoribonuclease Dicer (Gene name = Dicer1).
    The isoform 2, also known as t-Dicer, differs from the canonical sequence as follows: 1789-1922: LRRSEEDEEK...KANQPQVPNS → KSFLQMYPVP...TTGRSESLWK.
    The anti-Dicer ab14601, targeting Dicer specifically in the region between amino acids 1239 and 1255 of the PAZ domain, can therefore bind to both isoforms described on http://www.uniprot.org/uniprot/Q9UPY3.

    Read More

    Abcam Scientific Support

    Answered on Dec 20 2012

    Question

    Using ab14601 in ChIP and WB, human Hek 2 and Hek 3 samples. also mouse samples.
    Previous vials have worked for human. None have worked for mouse.
    Last 2 vials ordered have not worked at all: 15th Nov (order #####) and 3rd Oct (order #####)

    Read More

    Abcam community

    Verified customer

    Asked on Dec 10 2012

    Answer

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from the last 2 vials of this antibody.

    The details you have kindly provided will provide us with vital information for our monitoring of product quality

    I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results from the last 2 vials have not been successful.

    I traced the order number for the two vials that did not work:

    Order ##### Lot number GR97114-5
    Order ##### Lot number GR97114-8

    These were indeed from the same original lot number (GR97114). Unfortunately, I checked our system and I am very sorry to confirm we regrettably do not have any different lots in stock at the moment.

    I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch. Regrettably, I can suggest you have received bad vials on this occasion. Of course your enquiry will remain on our complaint records for continuing monitoring.

    Therefore, I would be pleased to provide two replacement vials from the same lot, which will still be covered by our guarantee. Alternatively, I fully understand your concerns and if you prefer a credit note in this case, I will be pleased to arrange this for you.

    I am sorry I have still not managed to trace your current order. I have alerted our customer service team to your request and asked them to let me know if they see the order coming through. However, we do have many orders coming through. Please let me know how you would like to proceed with this order, if you want to go ahead or cancel.

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Abcam Scientific Support

    Answered on Dec 10 2012

    Question

    Good day, I do not have a problem so much as a technical question about the DICER1 ab14601 monoclonal antibody. The technical datasheet specifies that the antibody is raised against a synthetic peptide with no further details about the peptide itself. While I understand the specific peptide sequence is likely proprietary, is there any additional info you could provide to me about the region of the protein targeted by the antibody? The reason I would like to know is that we are using the antibody on human tumors from patients who have germline DICER1 mutations and, sometimes, somatic mutations on the second allele in the tumor only. The mutations occur in a variety of locations in the protein and it is unclear what protein products should be expected, if any. Because we are dealing with patients there isn't always material available for testing beyond FFPE tumors, which limits the methods that can be used to answer our questions at the protein level. The antibody seems to work really weell and often gives beautiful, apparently specific staining. However, we have some tumors that stain positive and others that stain negative, and the results don't always appear consistent with what we think we know of the germline and somatic mutation status of the tumors, so there are concerns about the specificity of the antibody to recognize our mutant proteins. We are hoping that if we knew which part of the protein is being recognized by the antibody, it might help sort out our apparent contradictory results or formulate logical hypotheses to test and answer reviewers' questions. Thank you for any assistance you can provide.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 21 2012

    Answer

    Thank you for contacting Abcam.
    The peptide used to generate this antibody was located between amino acids *****of the human form of the protein (SwissProt: Q9UPY3 Human).
    Please let me know if there is anything else I can help you with, Free RabMab with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Nov 21 2012

    1-10 of 48 Abreviews or Q&A

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