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  1. Link

    dicer-antibody-4a6-ab82539.pdf

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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing RNAi
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Anti-Dicer antibody [4A6] (ab82539)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [4A6] (ab82539)
  • Western blot - Anti-Dicer antibody [4A6] (ab82539)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [4A6] (ab82539)

Key features and details

  • Mouse monoclonal [4A6] to Dicer
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Dicer antibody [4A6]
    See all Dicer primary antibodies
  • Description

    Mouse monoclonal [4A6] to Dicer
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Normal human ovary. Human placental tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: None
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    4A6
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Dicer
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab82539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/100 - 1/500. Predicted molecular weight: 217 kDa.
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Notes
WB
1/100 - 1/500. Predicted molecular weight: 217 kDa.
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Target

  • Function

    Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
  • Involvement in disease

    Pleuropulmonary blastoma
    Goiter multinodular 1, with or without Sertoli-Leydig cell tumors
    Rhabdomyosarcoma, embryonal, 2
    DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960).
  • Sequence similarities

    Belongs to the helicase family. Dicer subfamily.
    Contains 1 Dicer dsRNA-binding fold domain.
    Contains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 PAZ domain.
    Contains 2 RNase III domains.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q9UPY3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23405 Human
    • Omim: 606241 Human
    • SwissProt: Q9UPY3 Human
    • Unigene: 738957 Human
    • Unigene: 87889 Human
    • Alternative names

      • DCR antibody
      • DCR1 antibody
      • Dicer 1 ribonuclease III antibody
      • Dicer 1 ribonuclease type III antibody
      • Dicer antibody
      • DICER_HUMAN antibody
      • DICER1 antibody
      • Double-strand-specific ribonuclease antibody
      • Endoribonuclease Dicer antibody
      • Helicase MOI antibody
      • Helicase with RNase motif antibody
      • HERNA antibody
      • KIAA0928 antibody
      • MNG1 antibody
      • RMSE2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [4A6] (ab82539)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [4A6] (ab82539)
      ab82539, at 1/20 dilution, staining Dicer in normal human ovary by Immunohistochemistry using paraffin-embedded tissue.
    • Western blot - Anti-Dicer antibody [4A6] (ab82539)
      Western blot - Anti-Dicer antibody [4A6] (ab82539)
      ab82539 has been verified by siRNA target validation. The figure shows the elimination of the antigen band on western after treatment of cells with siRNA against human Dicer. No effect is seen on treatment with an irrelevant siRNA against GAPDH.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [4A6] (ab82539)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dicer antibody [4A6] (ab82539)
      ab82539, at 1/20 dilution, staining Dicer in human lung tumor by Immunohistochemistry using paraffin-embedded tissue.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (2)

    Publishing research using ab82539? Please let us know so that we can cite the reference in this datasheet.

    ab82539 has been referenced in 2 publications.

    • Ohno S  et al. Development of Novel Small Hairpin RNAs That do not Require Processing by Dicer or AGO2. Mol Ther 24:1278-89 (2016). PubMed: 27109632
    • Tan X  et al. A 5-microRNA signature for lung squamous cell carcinoma diagnosis and hsa-miR-31 for prognosis. Clin Cancer Res 17:6802-11 (2011). IHC-P ; Human . PubMed: 21890451

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