Key features and details
- Mouse monoclonal [N167/7] to Dicer
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-Dicer antibody [N167/7]
See all Dicer primary antibodies
DescriptionMouse monoclonal [N167/7] to Dicer
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- Rat brain normal tissue lysate - total protein (ab29475) can be used as a positive control in WB. ICC/IF: SK-N-BE cells.
The clone number has been updated from S167-7 to N167/7, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab167444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 217 kDa.|
FunctionDouble-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
Involvement in diseasePleuropulmonary blastoma
Goiter multinodular 1, with or without Sertoli-Leydig cell tumors
Rhabdomyosarcoma, embryonal, 2
DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960).
Sequence similaritiesBelongs to the helicase family. Dicer subfamily.
Contains 1 Dicer dsRNA-binding fold domain.
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 PAZ domain.
Contains 2 RNase III domains.
- Information by UniProt
- DCR antibody
- DCR1 antibody
- Dicer 1 ribonuclease III antibody
4% formaldehyde-fixed SK-N-BE cells labeling Dicer using ab167444 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/100 dilution incubated for 1 hour at room temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).
Lane 2 : Anti-Dicer antibody [N167/7] (ab167444) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Rat Brain Membrane lysate at 10 µg with 5% milk.
Lane 2 : Goat Anti-Mouse IgG at 1/200 dilution
Predicted band size: 217 kDa
Observed band size: 215 kDa why is the actual band size different from the predicted?
Color Development: TMB solution for 10 min at RT.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab167444 has been referenced in 1 publication.
- Liu D et al. Ago2 and Dicer1 are involved in METH-induced locomotor sensitization in mice via biogenesis of miRNA. Addict Biol 24:498-508 (2019). PubMed: 29516602