Anti-Digoxigenin antibody [21H8] (ab420)
Key features and details
- Mouse monoclonal [21H8] to Digoxigenin
- Suitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridization, Southern Blot
- Reacts with: Species independent
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-Digoxigenin antibody [21H8]
See all Digoxigenin primary antibodies -
Description
Mouse monoclonal [21H8] to Digoxigenin -
Host species
Mouse -
Specificity
Reacts with free and bound digoxigenin.
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Tested applications
Suitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridization, Southern Blotmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Digoxigenin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section. -
Storage buffer
Constituents: 1.2114% Tris, 0.7507% Glycine, 2% Sucrose -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
21H8 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. ab419 can be used for the detection of a diverse range of digoxigenin labelled compounds and in a variety of techniques
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ELISA |
1/1000 - 1/10000.
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IHC-P |
1/200 - 1/2000.
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IHC-Fr |
1/200 - 1/2000.
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In situ hybridization |
Use at an assay dependent concentration.
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Southern Blot |
1/500 - 1/5000.
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Notes |
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WB
1/500 - 1/5000. ab419 can be used for the detection of a diverse range of digoxigenin labelled compounds and in a variety of techniques |
ELISA
1/1000 - 1/10000. |
IHC-P
1/200 - 1/2000. |
IHC-Fr
1/200 - 1/2000. |
In situ hybridization
Use at an assay dependent concentration. |
Southern Blot
1/500 - 1/5000. |
Target
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Relevance
Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications. -
Alternative names
- 1672-46-4 antibody
- BRN 0096479 antibody
- DIG antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (40)
ab420 has been referenced in 40 publications.
- Qian F et al. MiR-378a-3p as a putative biomarker for hepatocellular carcinoma diagnosis and prognosis: Computational screening with experimental validation. Clin Transl Med 11:e307 (2021). PubMed: 33634974
- Mann EK et al. Associations between DNA Damage and PD-L1 Expression in Ovarian Cancer, a Potential Biomarker for Clinical Response. Biology (Basel) 10:N/A (2021). PubMed: 33946684
- Samsonova A et al. Lin28, a major translation reprogramming factor, gains access to YB-1-packaged mRNA through its cold-shock domain. Commun Biol 4:359 (2021). PubMed: 33742080
- Shibata T et al. Small molecule targeting r(UGGAA)n disrupts RNA foci and alleviates disease phenotype in Drosophila model. Nat Commun 12:236 (2021). PubMed: 33431896
- Cortes-Troncoso J et al. T cell exosome-derived miR-142-3p impairs glandular cell function in Sjögren's syndrome. JCI Insight 5:N/A (2020). PubMed: 32376798