Anti-Digoxigenin antibody [21H8] (ab420)
- Datasheet
- Specific References (15)
- Protocols
Overview
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Product nameAnti-Digoxigenin antibody [21H8]
See all Digoxigenin primary antibodies -
DescriptionMouse monoclonal [21H8] to Digoxigenin
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Host speciesMouse
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Tested applicationsSuitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridization, ICC/IF, Southern Blotmore details
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone number21H8
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Myelomaunknown
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/5000. ab419 can be used for the detection of a diverse range of digoxigenin labelled compounds and in a variety of techniques | |
| ELISA | 1/1000 - 1/10000. | |
| IHC-P | 1/200 - 1/2000. | |
| IHC-Fr | 1/200 - 1/2000. | |
| In situ hybridization | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 23508956 | |
| Southern Blot | 1/500 - 1/5000. |
Target
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RelevanceDigoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
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Alternative names
- 1672-46-4 antibody
- BRN 0096479 antibody
- DIG antibody
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Protocols
References
This product has been referenced in:
- Moradi M et al. Differential roles of a-, ß-, and ?-actin in axon growth and collateral branch formation in motoneurons. J Cell Biol 216:793-814 (2017). Read more (PubMed: 28246119) »
- Wang Y et al. Orientation and repositioning of chromosomes correlate with cell geometry-dependent gene expression. Mol Biol Cell 28:1997-2009 (2017). Read more (PubMed: 28615317) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"