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  1. Link

    digoxigenin-antibody-21h8-ab420.pdf

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Anti-Digoxigenin antibody [21H8] (ab420)

  • Datasheet
  • SDS
Submit a review Q&A (15)References (40)

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Promotion Information

Abpromise

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Key features and details

  • Mouse monoclonal [21H8] to Digoxigenin
  • Suitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridization, Southern Blot
  • Reacts with: Species independent
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alkaline Phosphatase FITC

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FITC Anti-Digoxigenin antibody [21H8] (ab119349)
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HRP Anti-Mouse Serum Albumin antibody (ab19195)
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Anti-CD3 zeta antibody [EP286Y] (ab40804)

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Overview

  • Product name

    Anti-Digoxigenin antibody [21H8]
    See all Digoxigenin primary antibodies
  • Description

    Mouse monoclonal [21H8] to Digoxigenin
  • Host species

    Mouse
  • Specificity

    Reacts with free and bound digoxigenin.

  • Tested applications

    Suitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridization, Southern Blotmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to Digoxigenin.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
  • Storage buffer

    Constituents: 1.2114% Tris, 0.7507% Glycine, 2% Sucrose
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    21H8
  • Myeloma

    unknown
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • Conjugates

Associated products

  • Alternative Versions

    • Alkaline Phosphatase Anti-Digoxigenin antibody [21H8] (ab119345)
    • FITC Anti-Digoxigenin antibody [21H8] (ab119349)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/5000. ab419 can be used for the detection of a diverse range of digoxigenin labelled compounds and in a variety of techniques
ELISA
1/1000 - 1/10000.
IHC-P
1/200 - 1/2000.
IHC-Fr
1/200 - 1/2000.
In situ hybridization
Use at an assay dependent concentration.
Southern Blot
1/500 - 1/5000.
Notes
WB
1/500 - 1/5000. ab419 can be used for the detection of a diverse range of digoxigenin labelled compounds and in a variety of techniques
ELISA
1/1000 - 1/10000.
IHC-P
1/200 - 1/2000.
IHC-Fr
1/200 - 1/2000.
In situ hybridization
Use at an assay dependent concentration.
Southern Blot
1/500 - 1/5000.

Target

  • Relevance

    Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
  • Alternative names

    • 1672-46-4 antibody
    • BRN 0096479 antibody
    • DIG antibody
    • EINECS 216-806-2 antibody
    • HSDB 7108 antibody
    • Lanadigenin antibody
    • Lanadigigenin antibody
    • ST056392 antibody
    see all

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (40)

Publishing research using ab420? Please let us know so that we can cite the reference in this datasheet.

ab420 has been referenced in 40 publications.

  • Qian F  et al. MiR-378a-3p as a putative biomarker for hepatocellular carcinoma diagnosis and prognosis: Computational screening with experimental validation. Clin Transl Med 11:e307 (2021). PubMed: 33634974
  • Mann EK  et al. Associations between DNA Damage and PD-L1 Expression in Ovarian Cancer, a Potential Biomarker for Clinical Response. Biology (Basel) 10:N/A (2021). PubMed: 33946684
  • Samsonova A  et al. Lin28, a major translation reprogramming factor, gains access to YB-1-packaged mRNA through its cold-shock domain. Commun Biol 4:359 (2021). PubMed: 33742080
  • Shibata T  et al. Small molecule targeting r(UGGAA)n disrupts RNA foci and alleviates disease phenotype in Drosophila model. Nat Commun 12:236 (2021). PubMed: 33431896
  • Cortes-Troncoso J  et al. T cell exosome-derived miR-142-3p impairs glandular cell function in Sjögren's syndrome. JCI Insight 5:N/A (2020). PubMed: 32376798
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 15 Abreviews or Q&A

Question

What buffer is the antibody provided in? Just PBS?

Read More

Abcam community

Verified customer

Asked on Sep 06 2012

Answer

Thank you very much for your call yesterday and for your patience while I've been in touch with the lab about this antibody.

The antibody is protein A purified then lyophilized from a buffer ofTris 0.1M, glycine 0.1M, sucrose 2%,andit is later re-suspended with sterile water.

I hope that this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

Read More

Abcam Scientific Support

Answered on Sep 06 2012

Question

Inquiry: Hi Could you please tell me what the overall charge of the antibody is at pH7? Thanks

Read More

Abcam community

Verified customer

Asked on Aug 22 2012

Answer

Thank you for contacting us.
Technically the charge on antibodies at pH7 should be neutral however it depends on antibody.
We do not have any information about charge for ab420 so I am sorry we are unable to share any information.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Aug 22 2012

Question

What is the concentration of this antibody?

Read More

Abcam community

Verified customer

Asked on Jul 10 2012

Answer

Thank you for contacting Abcam regarding ab420.


The antibody has previously been sold as an ascitic fluid (concentration not determined because not relevant).


More recently we have provided protein A purified antibody packaged as 100 ug in 200ul to reach a final concentration of 0.5 mg/ml.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Read More

Abcam Scientific Support

Answered on Jul 10 2012

Question

ich würde mich für einen Sekundärantikörper mit einem FITC Label entscheiden.
Dieser sollte folgenden Primärantikörper erkennen ab420 (Anti-Digoxigenin antibody).
Vielen Dank für Ihre Hilfe und Ihren Service.

Ich wünsche Ihnen ein schönes Wochenende!

Mit freundlichen Grüßen

Read More

Abcam community

Verified customer

Asked on Jul 09 2012

Answer

Vielen Dank für Ihre Antwort.

Ich kann Ihnen ein Röhrchen von einem der folgenden Antiköpern anbieten. Alle sind gegen Maus IgG1 gerichtet, FITC konjugiert und für Flow Cytometry getestet und garantiert:

ab97239

https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-FITC-ab97239.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-FITC-ab97239.html).

ab98692

https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-FITC-preadsorbed-ab98692.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-FITC-preadsorbed-ab98692.html).

ab133859

https://www.abcam.com/Rat-monoclonal-SB77e-Mouse-IgG1-HL-FITC-preadsorbed-ab133859.html (or use the following: https://www.abcam.com/Rat-monoclonal-SB77e-Mouse-IgG1-HL-FITC-preadsorbed-ab133859.html).

ab11588

https://www.abcam.com/Rat-monoclonal-LO-MG-1-2-Mouse-IgG1-heavy-chain-FITC-ab11588.html (or use the following: https://www.abcam.com/Rat-monoclonal-LO-MG-1-2-Mouse-IgG1-heavy-chain-FITC-ab11588.html).

Bitte lassen Sie mich wissen, welchen Antikörper Sie bevorzugen.

Read More

Abcam Scientific Support

Answered on Jul 09 2012

Question

in wie fern könnte ein unaufgereinigter Antikörper die Kopplung mit einem Sekundärantikörper be- bzw. verhindern.

In Folge weiterer Messungen musste ich feststellen, dass die Euphorie vorschnell war.

Mir gelingt es nicht, dass ich mit Hilfe des Durchflusszytometer einen Signal detektiere.



Ich schildere Ihnen zunächst noch einmal den Versuch:



Zunächst binden Beads und doppelsträngige Oligos.

Anschließend erfolgt die Bindung eines monoklonalen Antikörpers.

Daran sollte sich die Bindung des Sekundärantikörpers, welcher fluoreszenzmarkiert ist, anschließen.

Mittels Durchflusszytometer soll die Auswertung erfolgen.

Die Reaktionen finden in einem PBS Puffer statt. Und zwischen den einzelnen Bindungen werden Waschschritte durchgeführt.



Ich bin Neuling auf dem Gebiet der Antikörper und deren Eigenarten.

Durch einen anderen Versuch konnte ich bereits testen, dass die Kopplung von Beads + Oligo + Biotin-maskierter Antikörper + Fluoreszenzfarbstoff funktioniert.

Nun wurden der maskierter Antikörper und der Fluoreszenzfarbstoff durch den Primärantikörper und einen Sekundärantikörper ausgetauscht. Kein Signal wurde detektiert. Die Fluoreszenz wurde bereits zweifach überprüft. Sie ist im ausreichendem Maße vorhanden.



Haben Sie eine Idee, warum die Kopplung nicht funktioniert?





Mit freundlichen Grüßen

Read More

Abcam community

Verified customer

Asked on Jun 20 2012

Answer

Vielen Dank für Ihre Email. Es tut mir leid zu hören, dass Ihr versuch nicht optimal läuft.

Wenn ich Sie richtig verstehen haben sie den sekundären Antikörper (ab99900) mit einem anderen primären Antikörper ausprobiert? Und Sie konnten ein Signal sehen?

Das bedeutet, dass, wie Sie schon annehmen, die Bindung vom Sekundaren Antikörper an den primären nicht optimal ist. Ich schlage deshalb vor den sekundären weniger zu verdünnen.

Ich schlage auch vor eine Negativkontrolle durchzuführen für den ersten Versuch: Die Beads könnten unspezifisch an streptavidin (mit Fluoreszenz) binden und so ein falsch positive Ergebnisse zeigen. Eine negative Kontrolle ohne Antikörper könnte das aufdecken.

Manche sekundären Antikörper erkennen die verschiedene IgG Isotypen mit unterschiedlicher Affinität. Vielleicht können Sie sich einen andern sekundären Antikörper von einem Nachbarlabor ausleihen und diesen für einen Versuch verwenden?

Beide primären Antikörper sind bis jetzt nicht im FACS getestet worden.

Ich hoffe, diese Vorschläge helfen bei der Aufklärung. Bitte lassen sie mich wissen, wie die Versuche ausgehen.

Read More

Abcam Scientific Support

Answered on Jun 20 2012

Question

vielen Dank für Ihre Rückmeldung und die Beantwortung meiner Frage.

Die Kopplungsreaktion wurde mit folgendem Primärantikörper durchgeführt:



Anti-Digoxigenin antibody [21H8] (ab420)



Die Reaktion hat funktioniert und ich bekomme auswertbare Ergebnisse.

Vielen Dank für Ihren Hinweis. Eine Frage hätte ich noch. Auf dem Produktzettel kann

ich keine Angaben bezüglich der Konzentration entnehmen.

Könnten Sie mir diese mitteilen?



Mit freundlichen Grüßen

Read More

Abcam community

Verified customer

Asked on Jun 18 2012

Answer

Vielen Dank für Ihre Antwort.

Es freut mich zu hören, dass das Experiment mit einem unkonjugierten Antikörperfunktioniert hat.

Leider ist es nicht möglich die Konzentration von ab420 zu bestimmen. Bei ab420 handelt es sich um unaufgereinigtes Aszites. Die Konzentration von Antikörpern wird über die Proteinmenge bestimmt. Aszites enthält aber nicht nur den spezifischen Antikörper sondern auch andere Proteine und macht deshalb eine Konzentrationsbestimmung unmöglich.

Wir empfehlen 5mg/ml als Anhaltspunkt anzunehmen für Aszites.

Es tut mir leid, dass ich Ihnen keine genauere Angaben machen konnte und hoffe diese Information ist dennoch hilfreich.

Ich wünsche Ihnen weiterhin viel Erfolg mit Ihrer Forschung und verbleibe

Read More

Abcam Scientific Support

Answered on Jun 18 2012

Question

I am interested in your mouse monoclonal antidig antibody. I am now using sheep polyclonal. How much stronger your antibody-dig interaction as compared to polyclonal antibody?

Read More

Abcam community

Verified customer

Asked on Sep 23 2011

Answer

Thank you for contacting us. For a valid comparison, we would need to test the two antibodies together in the same assay on the same material. Sometimes a dissociation constant is calculated for antibodies and the antigens they bind to, which allows comparison of different antibodies, but this is rare, and the dissociation constant of ab420 has not been determined. For a very rough comparison, I suggest comparing the ranges of dilutions that are listed in the ab420 application notes with the dilutions you typically use with your sheep polyclonal. However, the suggested ab420 dilution ranges are quite broad: there is a 10-fold difference between the high and low suggestions for each assay type. I hope this is at least somewhat helpful. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Sep 23 2011

Question

Hi, I am looking to do some work using the anti-dig - dig labelled ssDNA interaction and I was hoping you could give me some information as to what would be a good hybridisation buffer to use. I need to use something which doesn't have a high salt concentration. Thanks

Read More

Abcam community

Verified customer

Asked on Feb 04 2009

Answer

Thank you for your enquiry. I have been in contact with the originator of ab420 Digoxigenin antibody [21H8] and I can confirm that this has been tested for this application (anti-dig-dig labeled ssDNA interaction) with a hybridisation buffer SSC 5X (NaCl 0,75M, Sodium citrate 75 mM, pH 7) and gave good results. However, I don't know if this buffer has the right concentration for you? I can suggest trying a lower concentration, though I am sorry we would not be able to provide any information on what the results would be on this occasion. I hope this is helpful to you. Should you have any further questions, please do not hesitate to contact us.

Read More

Abcam Scientific Support

Answered on Feb 04 2009

Question

Can you tell me if this product contains the Fc fragment?

Read More

Abcam community

Verified customer

Asked on Dec 05 2007

Answer

Thank you for your enquiry. I can confirm that this product contains the Fc fragment.

Read More

Abcam Scientific Support

Answered on Dec 05 2007

Question

Can you tell me what was used to make this antibody?

Read More

Abcam community

Verified customer

Asked on Aug 24 2007

Answer

Thank you for your enquiry. The hybridoma used to make this product was developed from mice as indicated on the data sheet. The immunogen (digoxigenin) was used following the standard protocol for the development of monoclonal antibody. Then fusion was made using splenocyte and myeloma SP2OAG. I hope this information will be useful.

Read More

Abcam Scientific Support

Answered on Aug 24 2007

1-10 of 15 Abreviews or Q&A

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