Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. ab419 can be used for the detection of a diverse range of digoxigenin labelled compounds and in a variety of techniques|
|ELISA||1/1000 - 1/10000.|
|IHC-P||1/200 - 1/2000.|
|IHC-Fr||1/200 - 1/2000.|
|In situ hybridization||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 23508956|
|Southern Blot||1/500 - 1/5000.|
RelevanceDigoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
- 1672-46-4 antibody
- BRN 0096479 antibody
- DIG antibody
This product has been referenced in:
- Yamazaki T et al. Functional Domains of NEAT1 Architectural lncRNA Induce Paraspeckle Assembly through Phase Separation. Mol Cell 70:1038-1053.e7 (2018). Read more (PubMed: 29932899) »
- Moradi M et al. Differential roles of a-, ß-, and ?-actin in axon growth and collateral branch formation in motoneurons. J Cell Biol 216:793-814 (2017). Read more (PubMed: 28246119) »