Overview

  • Product name

  • Description

    Chicken polyclonal to Digoxigenin
  • Host species

    Chicken
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chickens were immunised with Digoxigenin conjugated with KLH.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 - 2 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
    • Alternative names

      • 1672-46-4 antibody
      • BRN 0096479 antibody
      • DIG antibody
      • EINECS 216-806-2 antibody
      • HSDB 7108 antibody
      • Lanadigenin antibody
      • Lanadigigenin antibody
      • ST056392 antibody
      see all

    References

    This product has been referenced in:

    • Huang J  et al. The DNA translocase FANCM/MHF promotes replication traverse of DNA interstrand crosslinks. Mol Cell 52:434-46 (2013). Read more (PubMed: 24207054) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
    Fixative
    Formaldehyde

    Mr. Joseph Polex Wolf

    Verified customer

    Submitted Aug 15 2014

    Answer

    Thank you for contacting us. I am sorry to hear that you have encountered problems wit this antibody. According to the datasheet (https://www.abcam.com/digoxigenin-antibody-ab51946.html), the recommended storage temperature is 4 degrees, not -20 degrees. This is probably why the antibody was originally working well and is now not working anymore. We do strongly recommend following the storage instructions on the datasheet - they can vary from antibody to antibody based on composition of the storage buffer and type of antibody. If the antibody is stored correctly, it should be good for about 1 year (in case of 4 degree storage). Our guarantee is valid however for 6 months after the purchase:  We guarantee all our products for tested species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. 

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