• Product name

  • Description

    Sheep polyclonal to Digoxigenin
  • Host species

  • Specificity

    ab64509 detects Digoxigenin in synthetic or other in vitro samples.
  • Tested applications

    Suitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Digoxin-3-CMO conjugated to KLH.


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

  • Isotype

  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab64509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ELISA 1/100.
IHC-Fr Use at an assay dependent dilution.


  • Relevance

    Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
  • Alternative names

    • 1672-46-4 antibody
    • BRN 0096479 antibody
    • DIG antibody
    • EINECS 216-806-2 antibody
    • HSDB 7108 antibody
    • Lanadigenin antibody
    • Lanadigigenin antibody
    • ST056392 antibody
    see all


This product has been referenced in:

  • Natoli ME  et al. Paper-based detection of HIV-1 drug resistance using isothermal amplification and an oligonucleotide ligation assay. Anal Biochem 544:64-71 (2018). Read more (PubMed: 29229373) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for your enquiry. It has taken some time to obtain some information for you, and I apologise for the delay in responding and appreciate your patience.

I am sorry to confirm that as far as we are aware, none of our digoxigenin antibodies has never been tested with magnetic beads. All tested applications covered by our 6 month guarantee are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

We have the following two Digoxigenin antibodies which I hope may still be of interest to you. I can confirm these do not contain BSA, geletin or glycerol.

Digoxigenin antibody (ab76907)
Tested in: IHC-P, WB, ELISA
https://www.abcam.com/index.html?datasheet=76907 (or use the following: https://www.abcam.com/index.html?datasheet=76907).

Digoxigenin antibody (ab64509)
Tested in: WB, ELISA, IHC-Fr
https://www.abcam.com/index.html?datasheet=64509 (or use the following: https://www.abcam.com/index.html?datasheet=64509).

I am sorry we have no suitably tested antibodies covered by the guarantee for your application to suggeston this occasion. We like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:


I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

Read More


Thank you for contacting Abcam.

Although I do not know the reasons behind the different size offering, each product is guaranteed to react against Digoxigenin in WB and ELISA. At Abcam, providing quality service is just as important as providing quality products. Our Abpromise® ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund.
The Abcam Abpromise® guarantee:

100% Scientific and Customer Support for any product you buy from Abcam or one of our authorized distributors.

We guarantee our products work in the tested species and applications stated on the datasheet.

We will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase.

We investigate any quality concerns raised by customers to ensure our catalog contains products that perform to the highest standards.

Please note these conditions to our Abpromise®:

Protocol information must be provided in order for the claim to be validated.

Antibodies tested in recombinant samples only are not guaranteed for use on endogenous samples.

“Predicted to react” information on the datasheets is provided for reference only and these species are not guaranteed.

Fast Track antibodies are covered for ELISA against the immunizing peptide only.

I hope this information is helpful, but please do not hesitate to contact us with further questions.

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up