Overview

  • Product name

  • Description

    Goat polyclonal to Digoxigenin
  • Host species

    Goat
  • Specificity

    ab76907 reacts 100% with digoxigenin and digoxin, it does not cross-react with the carrier protein KLH or other steroids.
  • Tested applications

    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to Digoxigenin conjugated to keyhole limpet haemocyanin. Digoxigenin conjugated to KLH.

  • Positive control

    • Here are some suggestions: In IHC digoxigenin-labeled proteins: 1 µg/ml - 2 µg/ml. In WB digoxigenin-labeled proteins: 0.5 µg/ml – 2 µg/ml. In Situ hybridization: Digoxigenin-labeled nucleic acid 0.1 µg/ml - 0.8 µg/ml. The optimum dilution should be determined by the individual lab.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.87% Sodium chloride, 0.0268% PBS
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Purification notes

    ab76907 is purified from immunized serum by ion exchange chromatography and immunoaffinity chromatography
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/40000. Detects a band of approximately 150 kDa.
ELISA 1/100000 - 1/1e+006.

Digoxigenin-BSA at 2-5 µg/ml, apply 50 µl/well in ELISA.

Target

  • Relevance

    Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
  • Alternative names

    • 1672-46-4 antibody
    • BRN 0096479 antibody
    • DIG antibody
    • EINECS 216-806-2 antibody
    • HSDB 7108 antibody
    • Lanadigenin antibody
    • Lanadigigenin antibody
    • ST056392 antibody
    see all

References

This product has been referenced in:

  • Visser EWA  et al. Continuous biomarker monitoring by particle mobility sensing with single molecule resolution. Nat Commun 9:2541 (2018). Read more (PubMed: 29959314) »
  • Mutreja K  et al. ATR-Mediated Global Fork Slowing and Reversal Assist Fork Traverse and Prevent Chromosomal Breakage at DNA Interstrand Cross-Links. Cell Rep 24:2629-2642.e5 (2018). Read more (PubMed: 30184498) »
See all 5 Publications for this product

Customer reviews and Q&As

1-10 of 12 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde

Mr. Joseph Polex Wolf

Verified customer

Submitted Aug 10 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Specification
lung
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: proteinase K
Permeabilization
No
Blocking step
Blocking reagent (Roche) as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 06 2012

Answer

Thank you for your enquiry. It has taken some time to obtain some information for you, and I apologise for the delay in responding and appreciate your patience.

I am sorry to confirm that as far as we are aware, none of our digoxigenin antibodies has never been tested with magnetic beads. All tested applications covered by our 6 month guarantee are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

We have the following two Digoxigenin antibodies which I hope may still be of interest to you. I can confirm these do not contain BSA, geletin or glycerol.

Digoxigenin antibody (ab76907)
Tested in: IHC-P, WB, ELISA
https://www.abcam.com/index.html?datasheet=76907 (or use the following: https://www.abcam.com/index.html?datasheet=76907).

Digoxigenin antibody (ab64509)
Tested in: WB, ELISA, IHC-Fr
https://www.abcam.com/index.html?datasheet=64509 (or use the following: https://www.abcam.com/index.html?datasheet=64509).


I am sorry we have no suitably tested antibodies covered by the guarantee for your application to suggeston this occasion. We like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver, lung)
Specification
liver, lung
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Boehringer Blocking reagent as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 21 2012

Answer

This is fine! please submit abreview for IHC-P application.

The free antibody code will be valid only, when your abreview gets published. So please submit your Abreview we will publish it asap.

Read More

Answer

Thank you for your email.

Please submit the abreview under the more closer IHC-Fr application.

Please do not forget to provide summary of the application you have used in additional notes box. Once we receive the abreview we will then update the datasheet.

In case you experience difficulty in receiving your free antibody please let me know I will be happy to help!

Best wishes,

Read More

Answer

DISCOUNT CODE: XXXXXXXXXX
Expiration date: 22-08-2012

I am very pleased to hear you would like to use ab76907 in ISH (In situ hybridization)and send us an image of your results.

This code will give you 1 free [PRIMARY ANTIBODY/PROTEIN] before the expiration date. To redeem this offer, please submit an Abreview with the image for [SPECIES/APP] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

This product is to be used in an untested ISH application, so you are eligible for our testing discount program if the antibody has not yet been purchased. You can get more information about collaboration discount at;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More

Answer

Thank you for contacting us.

Indeed HRP conjugated secondary antibody can be used for target localization after insitu hybridization. Please check the PubMed literature there are many publications available where scientist have used HRP conjugated secondries for ISH.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

1-10 of 12 Abreviews or Q&A

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