Key features and details
- Mouse monoclonal [BT.21H8] to Digoxigenin (Biotin)
- Suitable for: WB, ELISA, IHC-P, IHC-Fr
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG1
Product nameAnti-Digoxigenin antibody [BT.21H8] (Biotin)
See all Digoxigenin primary antibodies
DescriptionMouse monoclonal [BT.21H8] to Digoxigenin (Biotin)
Tested applicationsSuitable for: WB, ELISA, IHC-P, IHC-Frmore details
Species reactivityReacts with: Species independent
Synthetic peptide corresponding to Digoxigenin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purification notesPurified biotin conjugate.
Light chain typekappa
Our Abpromise guarantee covers the use of ab419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.|
|ELISA||1/1000 - 1/10000.|
|IHC-P||1/200 - 1/2000.|
|IHC-Fr||1/200 - 1/2000.|
RelevanceDigoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
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ab419 has been referenced in 3 publications.
- Heine S et al. CNGA3: A Target of Spinal Nitric Oxide/cGMP Signaling and Modulator of Inflammatory Pain Hypersensitivity. J Neurosci 31:11184-92 (2011). IHC-FoFr . PubMed: 21813679
- Silva VA et al. Expression and cellular localization of microRNA-29b and RAX, an activator of the RNA-dependent protein kinase (PKR), in the retina of streptozotocin-induced diabetic rats. Mol Vis 17:2228-40 (2011). In situ hybridization . PubMed: 21897745
- Arendt T et al. Selective cell death of hyperploid neurons in Alzheimer's disease. Am J Pathol 177:15-20 (2010). In situ hybridization . PubMed: 20472889