Overview

  • Product name
    Anti-Digoxigenin antibody [HRP.21H8] (HRP)
    See all Digoxigenin primary antibodies
  • Description
    Mouse monoclonal [HRP.21H8] to Digoxigenin (HRP)
  • Host species
    Mouse
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, IHC-Fr, ICC/IF, In situ hybridizationmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    HRP.21H8
  • Myeloma
    unknown
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000.
ELISA 1/1000 - 1/10000.
IHC-P 1/200 - 1/2000. Use in combination with tyramide signal amplification.
IHC-Fr 1/200 - 1/2000. 1/200 - 1/2000. Use in combination with tyramide signal amplification.
ICC/IF Use at an assay dependent concentration. PubMed: 22393045
In situ hybridization Use at an assay dependent dilution. PubMed: 20019669

Target

  • Relevance
    Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
  • Alternative names
    • 1672-46-4 antibody
    • BRN 0096479 antibody
    • DIG antibody
    • EINECS 216-806-2 antibody
    • HSDB 7108 antibody
    • Lanadigenin antibody
    • Lanadigigenin antibody
    • ST056392 antibody
    see all

Images

  • ab6212 at a dilution of 1/1000 staining Digoxigenin from human pancreatic tissue sections by immunohistochemistry (frozen). The antibody was incubated with the tissue for 2 hours.

    This image is courtesy of an Abreview submitted by an anonymous researcher on 9 December 2005.

    See Abreview

References

This product has been referenced in:
  • Chen W  et al. Effects of Yangzheng Sanjie Decoction-containing serum mediated by microRNA-7 on cell proliferation and apoptosis in gastric cancer. Oncol Lett 15:3621-3629 (2018). Read more (PubMed: 29467883) »
  • Manjon C  et al. Unravelling the Molecular Determinants of Bee Sensitivity to Neonicotinoid Insecticides. Curr Biol 28:1137-1143.e5 (2018). Read more (PubMed: 29576476) »
See all 13 Publications for this product

Customer reviews and Q&As

1-10 of 16 Abreviews or Q&A

Answer

Thank you for contacting us.

I have attached the CoC for ab6212 to this email.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thanks so much for sending the protocol, data and schematic image. That is all very helpful to see.

From previous email, it was noted that lot GR40978-1 produced a linear curve while lots GR44124-3, GF63837-3 and GR63837-4 produced a plateau instead. Comparing the data provided using these different lots I am not able to see a significant difference between the raw OD readings from these different lots. All curves seem consistant, showing a minimum detection limit of about 1000ng/ml

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Answer

Thank you for your enquiry.

Regarding lots GR62763-3 and GR62763-4 for ab6212: These are treated as 2 different lots in our stock system. However we receive this antibody from an outside lab and, from this lab, GR62763-3 and GR62763-4 are not produced in separate production runs. They are part of the same production run.


I hope this helps. Please contact me again if you have any further questions.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 31 2009

Answer

Thank you for your patience in this matter. I have received a reply from the originator of ab6212. The concentration of this product is lot dependent, usually between 0.2 - 0.5 mg/ml. The concentration of our present lot is ~0.4 mg/ml. Please don't hesitate to contact us again if you have further questions.

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Question
Answer

Thank you for your enquiry. I have received word from the originator that they have not specifically tested this product in in situ hybridization. Please see their response below: This product was used successfully in IHC at the dilution of 1/1000. For in situ hybridization, we don't have any results, however we assume that many customers used it for this application. We recommend the dilution of 1/500 to 1/1000. I have removed ISH from the datasheet as a tested application; however, there doesn't seem to be any reason it won't work. Please let me know if you have any problems.

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Answer

Thank you for your patience. Regarding ab6212 (Digoxigenin antibody [HRP.21H8]), here is the requested information: antibody concentration: 0.3 mg/ml immunogen: digoxigenin coupled to KLH (details unavailable) I hope this information helps. Please don't hesitate to contact me again if you have further questions.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (in situ hybridization in pancreas)
Specification
in situ hybridization in pancreas
Fixative
Paraformaldehyde
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1

Abcam user community

Verified customer

Submitted Dec 09 2005

Question
Answer

Thank you for your recent enquiry regarding ab6212. The concentration of the lot 104388 is 0.43 mg/ml. Please let me know if you require further information,

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1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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