Overview

  • Product name
    Dihydroethidium (DHE), Blue fluorescent dye
  • Description
    Cell-permeable blue fluorescent dye. Redox indicator.
  • Biological description

    Cell-permeable blue fluorescent dye. Redox indicator. Loses fluorescence on oxidation to ethidium (ab141391). Detects superoxide radical in vivo.

  • Purity
    > 95%
  • CAS Number
    104821-25-2
  • Chemical structure
    Chemical Structure

Properties

  • Excitation
    392nm
  • Emission
    410nm
  • Chemical name
    5-Ethyl-6-phenyl-6H-phenanthridine-3,8-diamine
  • Molecular weight
    315.41
  • Molecular formula
    C21H21N3
  • PubChem identifier
    128682
  • Storage instructions
    Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview
    Soluble in DMSO to 100 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES
    CCN1C(C2=C(C=CC(=C2)N)C3=C1C=C(C=C3)N)C4=CC=CC=C4
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:
  • Almeida SA  et al. Exercise training reduces cardiac dysfunction and remodeling in ovariectomized rats submitted to myocardial infarction. PLoS One 9:e115970 (2014). Read more (PubMed: 25551214) »
  • Laurindo FR  et al. Assessment of superoxide production and NADPH oxidase activity by HPLC analysis of dihydroethidium oxidation products. Methods Enzymol 441:237-60 (2008). Read more (PubMed: 18554538) »
  • Peshavariya HM  et al. Analysis of dihydroethidium fluorescence for the detection of intracellular and extracellular superoxide produced by NADPH oxidase. Free Radic Res 41:699-712 (2007). Read more (PubMed: 17516243) »
See 1 Publication for this product

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