Dihydrofluorescein diacetate, Fluorescent intracellular oxidant probe (ab145351)

Overview

  • Product name
    Dihydrofluorescein diacetate, Fluorescent intracellular oxidant probe
  • Description
    Fluorescent intracellular oxidant probe. Redox sensor.
  • Biological description
    Fluorescent intracellular oxidant probe. Redox sensor. Achieves higher intracellular concentrations resulting in brighter intracellular labelling but is less bright extracellularly than dihydrorhodamine 123 (ab145260). Oxidizes to fluorescein primarily under H2O2.
  • Purity
    > 97%
  • CAS Number
    35340-49-9
  • Chemical structure
    Chemical Structure

Properties

  • Excitation
    490nm
  • Emission
    490nm
  • Chemical name
    2-(3,6-Diacetyloxy-9H-xanthen-9-yl)benzoic acid
  • Molecular weight
    418.40
  • Molecular formula
    C24H18O7
  • PubChem identifier
    629056
  • Storage instructions
    Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview
    Soluble in DMSO to 25 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES
    CC(=O)OC1=CC2=C(C=C1)C(C3=C(O2)C=C(C=C3)OC(=O)C)C4=CC=CC=C4C(=O)O
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:
  • Bennetts P  et al. Effects of ubiquinol with fluid resuscitation following haemorrhagic shock on rat lungs, diaphragm, heart and kidneys. Exp Physiol 99:1007-15 (2014). Read more (PubMed: 24860150) »
  • Chen C  et al. Effects of Mitochondrial ATP-Sensitive Potassium Channels on the Proliferation and Secretion of Human Airway Smooth Muscle Cells. Iran J Allergy Asthma Immunol 13:420-7 (2014). Read more (PubMed: 25148800) »
  • Hempel SL  et al. Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic Biol Med 27:146-59 (1999). Read more (PubMed: 10443931) »

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab145351.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"
For licensing inquiries, please contact partnerships@abcam.com

Sign up