Product nameAnti-Dihydrofolate reductase (DHFR) antibody
See all Dihydrofolate reductase (DHFR) primary antibodies
DescriptionRabbit polyclonal to Dihydrofolate reductase (DHFR)
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Hamster, Human
Predicted to work with: Mouse, Rat, Cow, Pig
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
Concentration information loading...
Purification notesThis antibody is affinity isolated.
Our Abpromise guarantee covers the use of ab49881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 21 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
FunctionKey enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
PathwayCofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Sequence similaritiesBelongs to the dihydrofolate reductase family.
Contains 1 DHFR (dihydrofolate reductase) domain.
- Information by UniProt
- DHFR antibody
- DHFRP1 antibody
- Dihydrofolate reductase antibody
All lanes : Anti-Dihydrofolate reductase (DHFR) antibody (ab49881) at 0.5 µg/ml
Lane 1 : recombinant DHFR at 0.2 µg
Lane 2 : recombinant DHFR at 0.1 µg
Lane 3 : recombinant DHFR at 0.05 µg
Lane 4 : recombinant DHFR at 0.025 µg
Lane 5 : recombinant DHFR at 0.0125 µg
Developed using the ECL technique.
Predicted band size: 21 kDa
Observed band size: 21 kDa
ab49881 at a 1/30 dilution staining Dihydrofolate reductase (DHFR) in human squamous cell carcinoma tissue by Immunohistochemistry/ Formalin fixed, paraffin embedded, incubated for 12 hours at 4°C. Heat mediated antigen retrieval step performed using EDTA pH 9.0. Blocked with 1% milk for 30 minutes at 25°C. Secondary used undiluted polyclonal Goat anti-rabbit IgG conjugated to biotin.
This product has been referenced in:
- Lee YH et al. DHFR and MDR1 upregulation is associated with chemoresistance in osteosarcoma stem-like cells. Oncol Lett 14:171-179 (2017). Read more (PubMed: 28693150) »
- Nakano M et al. A-to-I RNA Editing Up-regulates Human Dihydrofolate Reductase in Breast Cancer. J Biol Chem 292:4873-4884 (2017). Read more (PubMed: 28188287) »