Overview

  • Product name

    Anti-Dihydrofolate reductase (DHFR) antibody
    See all Dihydrofolate reductase (DHFR) primary antibodies
  • Description

    Rabbit polyclonal to Dihydrofolate reductase (DHFR)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Dihydrofolate reductase (DHFR) aa 50-150 (internal sequence). The exact sequence is proprietary.
    Database link: P00374

  • Positive control

    • Breast Carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab82171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
  • Pathway

    Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
  • Sequence similarities

    Belongs to the dihydrofolate reductase family.
    Contains 1 DHFR (dihydrofolate reductase) domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • DHFR antibody
    • DHFRP1 antibody
    • Dihydrofolate reductase antibody
    • DYR antibody
    • DYR_HUMAN antibody
    • EC 1.5.1.3 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded section of Human breast carcinoma stained with ab82171 at 1/50 dilution. Incubate primary antibody (ab82171) for 30 minutes at RT.
  • ICC/IF image of ab82171 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82171, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Uramoto H  et al. TS, DHFR and GARFT expression in non-squamous cell carcinoma of NSCLC and malignant pleural mesothelioma patients treated with pemetrexed. Anticancer Res 30:4309-15 (2010). IHC ; Human . Read more (PubMed: 21036757) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry.

I have reviewed the immunogen sequences of the DHFR antibodies we have and regrettably the alignment with the e coli amino acid sequence is very low. Therefore, we would not anticipate that these antibodies would detect the DHFR in e coli. In this case, I am sorry we are not able to offer the AbTrial.

I am sorry there are no suitable DHFR antibodies to suggest to you on this occasion.

We are happy to be able to offer custom antibody services through Epitomics, an Abcam company. This may be helpful to you. For more information about these services visit:

http://www.epitomics.com/services/
email: service@epitomics.com

Alternatively, please contact Li Li at Epitomics:

li.li@epitomics.com

Alternatively, I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are:

www.biocompare.com
http://www.biocompare.com/ProductCategories/2045/Antibodies.html

In regard to the expiry date of the code you have, I can extend this to 31st December.

Please quote our correspondence reference number (CCE 4394684) when you place your order so that the person taking your order can check the records to see I have extended this for you.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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