Key features and details
- Rabbit polyclonal to Dihydrofolate reductase (DHFR)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Dihydrofolate reductase (DHFR) antibody
See all Dihydrofolate reductase (DHFR) primary antibodies
DescriptionRabbit polyclonal to Dihydrofolate reductase (DHFR)
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Dihydrofolate reductase (DHFR) aa 50-150 (internal sequence). The exact sequence is proprietary.
Database link: P00374
- Breast Carcinoma
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab82171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionKey enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
PathwayCofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Sequence similaritiesBelongs to the dihydrofolate reductase family.
Contains 1 DHFR (dihydrofolate reductase) domain.
- Information by UniProt
- DHFR antibody
- DHFRP1 antibody
- Dihydrofolate reductase antibody
Formalin-fixed, paraffin-embedded section of Human breast carcinoma stained with ab82171 at 1/50 dilution. Incubate primary antibody (ab82171) for 30 minutes at RT.
ICC/IF image of ab82171 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82171, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab82171 has been referenced in 2 publications.
- Wang M et al. Dihydrofolate reductase as a predictor for poor response to platinum-based chemotherapy in epithelial ovarian cancer. Int J Clin Exp Pathol 12:1723-1730 (2019). PubMed: 31933990
- Uramoto H et al. TS, DHFR and GARFT expression in non-squamous cell carcinoma of NSCLC and malignant pleural mesothelioma patients treated with pemetrexed. Anticancer Res 30:4309-15 (2010). IHC ; Human . PubMed: 21036757