Recombinant
RabMAb

Recombinant Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546)

Overview

  • Product name

    Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284]
    See all Dihydrofolate reductase (DHFR) primary antibodies
  • Description

    Rabbit monoclonal [EPR5284] to Dihydrofolate reductase (DHFR)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. corresponding to Human Dihydrofolate reductase (DHFR) aa 1-100.
    Database link: P00374

  • Positive control

    • HeLa, Jurkat, U937, 293T, C6, RAW 264.7 and PC-12 cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546) at 1/10000 dilution (purified)

      Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 2 : U937 (Human histiocytic lymphoma cell line) whole cell lysate
      Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
      Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
      Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 21 kDa
      Observed band size: 21 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5284] (ab133546) at 1/1000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : U937 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 21 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Gong L  et al. The mevalonate coordinates energy input and cell proliferation. Cell Death Dis 10:327 (2019). Read more (PubMed: 30975976) »
    • Ashkavand Z  et al. Metabolic Reprogramming by Folate Restriction Leads to a Less Aggressive Cancer Phenotype. Mol Cancer Res 15:189-200 (2017). WB ; Mouse . Read more (PubMed: 28108628) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133546.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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