Recombinant
RabMAb

Recombinant Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814)

Overview

  • Product name

    Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]
    See all Dihydrofolate reductase (DHFR) primary antibodies
  • Description

    Rabbit monoclonal [EPR5285] to Dihydrofolate reductase (DHFR)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Dihydrofolate reductase (DHFR) aa 150-250. The exact sequence is proprietary.

  • Positive control

    • HeLa, Jurkat, Y-79, 293T, Mouse spleen and Rat kidney lysates; Human cervical carcinoma tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC 1/25 - 1/50.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    Images

    • All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814) at 1/10000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : Jurkat cell lysates
      Lane 3 : Y-79 cell lysates
      Lane 4 : 293T cell lysates
      Lane 5 : Mouse spleen lysates
      Lane 6 : Rat kidney lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 21 kDa

    • ab124814 at 1/100 dilution, staining Dihydrofolate reductase (DHFR) in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.

    References

    This product has been referenced in:

    • Shinojima N  et al. Clinical significance of polyglutamylation in primary central nervous system lymphoma. Acta Neuropathol Commun 6:15 (2018). WB . Read more (PubMed: 29475458) »
    • Hughes L  et al. An active second dihydrofolate reductase enzyme is not a feature of rat and mouse, but they do have activity in their mitochondria. FEBS Lett 589:1855-62 (2015). WB ; Rat . Read more (PubMed: 25980602) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer



    This antibody was raised using a synthetic peptide corresponding to the C terminal end of the protein DHFR.

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    Answer

    Thank you for contacting Abcam regarding ab124814.


    For this product we do not have any information regarding storage at 4C. In general we would expect decreased activity over time when stored at this temperature. Furthermore, we do not have any additional data regarding stability beyond 12 months, but products stored at -20C are often stable for up to 5 years.


    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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