Product nameAnti-Dihydrofolate reductase (DHFR) antibody [EPR5285]
See all Dihydrofolate reductase (DHFR) primary antibodies
DescriptionRabbit monoclonal [EPR5285] to Dihydrofolate reductase (DHFR)
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Dihydrofolate reductase (DHFR) aa 150-250. The exact sequence is proprietary.
- HeLa, Jurkat, Y-79, 293T, Mouse spleen and Rat kidney lysates; Human cervical carcinoma tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 21 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)|
|ICC||1/25 - 1/50.|
FunctionKey enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
PathwayCofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Sequence similaritiesBelongs to the dihydrofolate reductase family.
Contains 1 DHFR (dihydrofolate reductase) domain.
- Information by UniProt
- DHFR antibody
- DHFRP1 antibody
- Dihydrofolate reductase antibody
All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814) at 1/10000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : Y-79 cell lysates
Lane 4 : 293T cell lysates
Lane 5 : Mouse spleen lysates
Lane 6 : Rat kidney lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 21 kDa
ab124814 at 1/100 dilution, staining Dihydrofolate reductase (DHFR) in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.
This product has been referenced in:
- Shinojima N et al. Clinical significance of polyglutamylation in primary central nervous system lymphoma. Acta Neuropathol Commun 6:15 (2018). WB . Read more (PubMed: 29475458) »
- Hughes L et al. An active second dihydrofolate reductase enzyme is not a feature of rat and mouse, but they do have activity in their mitochondria. FEBS Lett 589:1855-62 (2015). WB ; Rat . Read more (PubMed: 25980602) »