Dihydroxyacetone Phosphate (DHAP) Assay Kit (Fluorometric) (ab197003)

Overview

  • Product name

    Dihydroxyacetone Phosphate (DHAP) Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells
  • Assay type

    Quantitative
  • Sensitivity

    > 0.5 µM
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Dihydroxyacetone Phosphate (DHAP) Assay Kit (Fluorometric) (ab197003) is suitable for measuring low levels of DHAP typically found in a variety of samples. In this kit, triose phosphate isomerase (TPI) converts dihydroxyacetone phosphate (DHAP) to glyceraldehyde-3-phosphate (GAP), which subsequently undergoes a series of reaction and reduces the probe to generate fluorescence. The fluorescence intensity generated is directly proportional to the amount of dihydroxyacetone Phosphate.

    Detection limit: 0.5 µM DHAP.

  • Notes

    Dihydroxyacetone Phosphate (DHAP) is an important intermediate in both lipid biosynthesis and glycolysis. In glycolysis, fructose-1,6- diphosphate is converted to dydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GAP) by aldolase. Both DHAP and GAP serve as the intracellular pool for  triose phosphate. DHAP can be further converted into GAP by Triose Phosphate Isomerase (TPI).

    In humans, TPI deficiency is a rare autosomal disease. It causes hemolytic anemia, neurological diseases, and even death due to blockage of the glycolytic pathway and accumulation of DHAP in erythrocytes.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    DHAP Assay Buffer 1 x 25ml
    DHAP Developer 1 vial
    DHAP Enzyme Mix 1 vial
    DHAP Standard 1 vial
    PicoProbe Blue 1 x 400µl

Images

  • Typical DHAP Standard Curve obtained following assay protocol.

  • Measurement of DHAP level in a varity of samples: Jurkat (250 µg),and MCF-7 (150 µg) cell lysate, and in rat liver (50 µg) and rat muscle (150 µg) tissue lysate.

Protocols

References

ab197003 has not yet been referenced specifically in any publications.

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