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    dihydroxyacetone-phosphate-dhap-assay-kitfluorometric-ab197003.pdf

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Dihydroxyacetone Phosphate (DHAP) Assay Kit (Fluorometric) (ab197003)

  • Datasheet
  • SDS
  • Protocol Booklet
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Functional Studies - Dihydroxyacetone Phosphate (DHAP) Assay Kit (Fluorometric) (ab197003)
  • Functional Studies - Dihydroxyacetone Phosphate (DHAP) Assay Kit (Fluorometric) (ab197003)

Key features and details

  • Assay type: Quantitative
  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Sample type: Adherent cells, Other biological fluids, Plasma, Serum, Suspension cells, Tissue
  • Sensitivity: 0.5 µM

Overview

  • Product name

    Dihydroxyacetone Phosphate (DHAP) Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells
  • Assay type

    Quantitative
  • Sensitivity

    > 0.5 µM
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Dihydroxyacetone Phosphate (DHAP) Assay Kit (Fluorometric) (ab197003) is suitable for measuring low levels of DHAP typically found in a variety of samples. In this kit, triose phosphate isomerase (TPI) converts dihydroxyacetone phosphate (DHAP) to glyceraldehyde-3-phosphate (GAP), which subsequently undergoes a series of reaction and reduces the probe to generate fluorescence. The fluorescence intensity generated is directly proportional to the amount of dihydroxyacetone Phosphate.


    Detection limit: 0.5 µM DHAP.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K673 PicoProbe™ Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit. K673-100 is the same size as the 100 test size of ab197003.

    Dihydroxyacetone Phosphate (DHAP) is an important intermediate in both lipid biosynthesis and glycolysis. In glycolysis, fructose-1,6- diphosphate is converted to dydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GAP) by aldolase. Both DHAP and GAP serve as the intracellular pool for  triose phosphate. DHAP can be further converted into GAP by Triose Phosphate Isomerase (TPI).

    In humans, TPI deficiency is a rare autosomal disease. It causes hemolytic anemia, neurological diseases, and even death due to blockage of the glycolytic pathway and accumulation of DHAP in erythrocytes.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    DHAP Assay Buffer 1 x 25ml
    DHAP Developer 1 vial
    DHAP Enzyme Mix 1 vial
    DHAP Standard 1 vial
    PicoProbe Blue 1 x 400µl

Images

  • Functional Studies - Dihydroxyacetone Phosphate (DHAP) Assay Kit (Fluorometric) (ab197003)
    Functional Studies - Dihydroxyacetone Phosphate (DHAP) Assay Kit (Fluorometric) (ab197003)

    Typical DHAP Standard Curve obtained following assay protocol.

  • Functional Studies - Dihydroxyacetone Phosphate (DHAP) Assay Kit (Fluorometric) (ab197003)
    Functional Studies - Dihydroxyacetone Phosphate (DHAP) Assay Kit (Fluorometric) (ab197003)

    Measurement of DHAP level in a varity of samples: Jurkat (250 µg),and MCF-7 (150 µg) cell lysate, and in rat liver (50 µg) and rat muscle (150 µg) tissue lysate.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab197003? Please let us know so that we can cite the reference in this datasheet.

ab197003 has been referenced in 2 publications.

  • Parvaiz N  et al. Discovery of beta-lactamase CMY-10 inhibitors for combination therapy against multi-drug resistant Enterobacteriaceae. PLoS One 16:e0244967 (2021). PubMed: 33449932
  • Jiang H  et al. The Mitochondria-Targeted Metabolic Tubular Injury in Diabetic Kidney Disease. Cell Physiol Biochem 52:156-171 (2019). PubMed: 30816665

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