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Rabbit polyclonal to DIP13B - C-terminal
Recombinant fragment corresponding to a region within C terminal amino acids 476 and 664 of Human DIP13B (Uniprot ID: Q8NEU8)
- NT2D1 or IMR32 whole cell lysate; A431 cells; HBL435 xenograft
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 74 kDa.
||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
||1/100 - 1/1000.
Required for the regulation of cell proliferation in response to extracellular signals mediated by an early endosomal compartment. Links Rab5 to nuclear signal transduction.
High levels in brain, heart, kidney and skeletal muscle.
Involvement in disease
Note=A chromosomal aberration involving APPL2/DIP13B is found in patients with chromosome 22q13.3 deletion syndrome. Translocation t(12;22)(q24.1;q13.3) with SHANK3/PSAP2.
Contains 1 PH domain.
Contains 1 PID domain.
Early endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
Information by UniProt
- Adapter protein containing PH domain antibody
- Adapter protein containing PH domain PTB domain and leucine zipper motif 2 antibody
- adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 antibody
Western blot - Anti-DIP13B antibody - C-terminal (ab154661)
All lanes : Anti-DIP13B antibody - C-terminal (ab154661) at 1/1000 dilution
Lane 1 : NT2D1 whole cell lysate
Lane 2 : IMR32 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 74 kDa
7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-DIP13B antibody - C-terminal (ab154661)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling DIP13B using ab154661 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIP13B antibody - C-terminal (ab154661)
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, labeling DIP13B using ab154661 at 1/100 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"