Overview

  • Product name

    Anti-DIP13B/APPL2 antibody
    See all DIP13B/APPL2 primary antibodies
  • Description

    Rabbit polyclonal to DIP13B/APPL2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human DIP13B/APPL2 aa 476-664 (C terminal).
    Database link: Q8NEU8

  • Positive control

    • NT2D1 or IMR32 whole cell lysate; A431 cells; HBL435 xenograft
  • General notes

     This product was previously labelled as DIP13B

     

Properties

Applications

Our Abpromise guarantee covers the use of ab154661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 74 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Required for the regulation of cell proliferation in response to extracellular signals mediated by an early endosomal compartment. Links Rab5 to nuclear signal transduction.
  • Tissue specificity

    High levels in brain, heart, kidney and skeletal muscle.
  • Involvement in disease

    Note=A chromosomal aberration involving APPL2/DIP13B is found in patients with chromosome 22q13.3 deletion syndrome. Translocation t(12;22)(q24.1;q13.3) with SHANK3/PSAP2.
  • Sequence similarities

    Contains 1 PH domain.
    Contains 1 PID domain.
  • Cellular localization

    Early endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adapter protein containing PH domain antibody
    • Adapter protein containing PH domain PTB domain and leucine zipper motif 2 antibody
    • adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 antibody
    • APLP2 antibody
    • Appl2 antibody
    • DCC interacting protein 13 beta antibody
    • DCC-interacting protein 13-beta antibody
    • Dip13 beta antibody
    • Dip13-beta antibody
    • DP13B_HUMAN antibody
    • PTB domain and leucine zipper motif 2 antibody
    see all

Images

  • All lanes : Anti-DIP13B/APPL2 antibody (ab154661) at 1/1000 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : IMR32 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 74 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling DIP13B/APPL2 using ab154661 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

  • Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, labeling DIP13B/APPL2 using ab154661 at 1/100 dilution.

References

ab154661 has not yet been referenced specifically in any publications.

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