Key features and details
- Rabbit polyclonal to DIP13B/APPL2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DIP13B/APPL2 antibody
See all DIP13B/APPL2 primary antibodies
DescriptionRabbit polyclonal to DIP13B/APPL2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human DIP13B/APPL2 aa 476-664 (C terminal).
Database link: Q8NEU8
- NT2D1 or IMR32 whole cell lysate; A431 cells; HBL435 xenograft
This product was previously labelled as DIP13B
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 74 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.|
|ICC/IF||1/100 - 1/1000.|
FunctionRequired for the regulation of cell proliferation in response to extracellular signals mediated by an early endosomal compartment. Links Rab5 to nuclear signal transduction.
Tissue specificityHigh levels in brain, heart, kidney and skeletal muscle.
Involvement in diseaseNote=A chromosomal aberration involving APPL2/DIP13B is found in patients with chromosome 22q13.3 deletion syndrome. Translocation t(12;22)(q24.1;q13.3) with SHANK3/PSAP2.
Sequence similaritiesContains 1 PH domain.
Contains 1 PID domain.
Cellular localizationEarly endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
- Information by UniProt
- Adapter protein containing PH domain antibody
- Adapter protein containing PH domain PTB domain and leucine zipper motif 2 antibody
- adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 antibody
All lanes : Anti-DIP13B/APPL2 antibody (ab154661) at 1/1000 dilution
Lane 1 : NT2D1 whole cell lysate
Lane 2 : IMR32 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 74 kDa
7.5% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling DIP13B/APPL2 using ab154661 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, labeling DIP13B/APPL2 using ab154661 at 1/100 dilution.
ab154661 has not yet been referenced specifically in any publications.