Overview

  • Product name
    Anti-Diphtheria Toxin antibody [11D9]
    See all Diphtheria Toxin primary antibodies
  • Description
    Mouse monoclonal [11D9] to Diphtheria Toxin
  • Host species
    Mouse
  • Specificity
    ab51882 reacts with Diphtheria toxoid (formaldehyde inactivated Diphtheria toxin).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Immunogen

    Diphtheria toxoid (formaldehyde inactivated Diphtheria toxin).

  • General notes

    For a recombinant antibody to Diphtheria Toxin, please see - ab209329.

Properties

Applications

Our Abpromise guarantee covers the use of ab51882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 63 kDa (predicted molecular weight: 58 kDa).
ELISA 1/4000.

Target

  • Relevance
    Corynebacterium diphtheriae is a gram-positive, nonmotile bacteria found in soil and animal feces. C. diphteriae infect the epithelial cells of the upper respiratory tract from where they produce and secrete a potent toxin. This toxin is absorbed and disseminated through lymph channels and blood to the susceptible tissues of the body.
  • Cellular localization
    Secreted
  • Alternative names
    • Corynebacterium diphtheriae toxoid antibody
    • Diphtheria Toxin antibody
    • tox antibody

References

This product has been referenced in:
  • Schmohl JU  et al. Development of a Deimmunized Bispecific Immunotoxin dDT2219 against B-Cell Malignancies. Toxins (Basel) 10:N/A (2018). Read more (PubMed: 29316610) »

See 1 Publication for this product

Publishing research using ab51882? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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4 reviews or Q&A

10
Votes: Highest First

Thank you for getting back to me with your suggestions, Unfortunately I have already tried the things you have suggested. We actually need a minimum of 20ug of sample to bind so smaller amounts of protein are not going to work for us.

Thanks...

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Thank you very much for your reply.

I am sorry that these suggestions are not more helpful. If you would like to try a different DT antibody please let me know and I can arrange a testing discount for you.

If there is anything else ...

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I have attached my answers to your previous e-mail. See below.

Thank you

Thank you very much for your reply and for sending this additional protocol information and the images.

I've looked through the images and alsodiscussed the situation with some of my colleagues. Based on these images, it looks like the antibo...

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I have a question regarding your Immunoprecipitation protocol and looking for some advice. We are looking to bind CRM197 which also contains Vi antigen, basically we are hoping to bind all of the CRM197 so we can detect the Vi.

What we have ...

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Thank you very much for contacting us with your questions and for sending the details of your protocol.

I do have a few additional questions just to make sure that I understand the situation:

1) How is the CRM197 prepared prior to t...

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Order Details Antibody code: ab34420 Problem Choose: Problem with standard curve No signal or weak signal High background problem with standard curve and high background Lot number ab34420 (mAb anti-DT)  lot.877395 ab51882 (pAb-HRP anti DT) lot. 949834...

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Thank you for your reply and for taking the time to complete our questionnaire. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality. Reviewing this case,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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